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http://hdl.handle.net/1843/55123| Tipo: | Artigo de Periódico |
| Título: | Using Next-Generation Sequencing (NGS) to uncover diversity of wood-decaying fungi in neotropical atlantic forests |
| Título(s) alternativo(s): | Usando Sequenciamento de Nova Geração (NGS) para descobrir a diversidade de fungos decompositores de madeira em florestas atlânticas neotropicais |
| Autor(es): | Aline Bruna Martins Vaz Paula Luize Camargos Fonseca Laura Rabelo Leite Fernanda Badotti Anna Cristina de Matos Salim Flávio Marcos Gomes Araújo Sara Cuadros Orellana Ângelo Amâncio Duarte Carlos Augusto Rosa Guilherme Oliveira Aristóteles Góes Neto |
| Resumo: | A targeted amplicon-based metagenomics approach (metabarcoding) provides detailed access to the diversity of the mycobiome in any substrate in distinct environments on Earth. Fungi are the main decomposers of lignocellulosic woody debris in terrestrial forested ecosystems, contributing significantly to the global carbon cycle. The main objectives of this study were to assess the fungal taxonomic diversity in fallen woody debris samples from two Neotropical forest fragments (rainforest and seasonal forest), to analyze the qualitative and quantitative components of the taxonomic diversity, and to investigate the functional diversity of the ecological groups detected. Our study comprised three main methodological steps: (i) sampling in the field; (ii) extraction of DNA, amplification of targeted segments and massively parallel sequencing; and (iii) data analysis and interpretation. A total of 110 molecular operational taxonomic units showing sequence similarity of 95% or more across the two collection sites using two DNA metabarcoding markers (ITS1 and ITS2) were assigned to putative fungal genera in 59 families, 27 orders, and 3 phyla. The number of putative fungal genera and the relative abundance of reads for each genus are higher in the tropical rainforest site than in the tropical seasonal forest site. Most of the identified genera are ligninolytic and cellulolytic and/or hemicellulolytic Basidiomycota (Agaricomycetes) and Ascomycota (Sordariomycetes), but “sugar fungi” and fungi associated with plants and detritivorous insects were also detected. This is the first study using NGS as a rapid and large-scale useful strategy to uncover the diversity of wood-decaying fungi in tropical forests. |
| Assunto: | Metagenômica Madeira-microbiologia Floresta Úmida Florestas tropicais |
| Idioma: | eng |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Instituição: | UFMG |
| Departamento: | ICB - DEPARTAMENTO DE MICROBIOLOGIA |
| Tipo de Acesso: | Acesso Aberto |
| Identificador DOI: | http://dx.doi.org/10.11646/phytotaxa.295.1.1 |
| URI: | http://hdl.handle.net/1843/55123 |
| Data do documento: | 2017 |
| metadata.dc.url.externa: | https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.295.1.1 |
| metadata.dc.relation.ispartof: | Phytotaxa |
| Aparece nas coleções: | Artigo de Periódico |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| USING Next-Generation Sequencing (NGS) TO UNCOVER DIVERSITY OF WOOD-DECAYING FUNGI IN NEOTROPICAL ATLANTIC FORESTS.pdf | 7.21 MB | Adobe PDF | Visualizar/Abrir |
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