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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/55123
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dc.creatorAline Bruna Martins Vazpt_BR
dc.creatorPaula Luize Camargos Fonsecapt_BR
dc.creatorLaura Rabelo Leitept_BR
dc.creatorFernanda Badottipt_BR
dc.creatorAnna Cristina de Matos Salimpt_BR
dc.creatorFlávio Marcos Gomes Araújopt_BR
dc.creatorSara Cuadros Orellanapt_BR
dc.creatorÂngelo Amâncio Duartept_BR
dc.creatorCarlos Augusto Rosapt_BR
dc.creatorGuilherme Oliveirapt_BR
dc.creatorAristóteles Góes Netopt_BR
dc.date.accessioned2023-06-19T20:05:04Z-
dc.date.available2023-06-19T20:05:04Z-
dc.date.issued2017-
dc.citation.volume295pt_BR
dc.citation.issue1pt_BR
dc.identifier.doihttp://dx.doi.org/10.11646/phytotaxa.295.1.1pt_BR
dc.identifier.issn1179-3163pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/55123-
dc.description.resumoA targeted amplicon-based metagenomics approach (metabarcoding) provides detailed access to the diversity of the mycobiome in any substrate in distinct environments on Earth. Fungi are the main decomposers of lignocellulosic woody debris in terrestrial forested ecosystems, contributing significantly to the global carbon cycle. The main objectives of this study were to assess the fungal taxonomic diversity in fallen woody debris samples from two Neotropical forest fragments (rainforest and seasonal forest), to analyze the qualitative and quantitative components of the taxonomic diversity, and to investigate the functional diversity of the ecological groups detected. Our study comprised three main methodological steps: (i) sampling in the field; (ii) extraction of DNA, amplification of targeted segments and massively parallel sequencing; and (iii) data analysis and interpretation. A total of 110 molecular operational taxonomic units showing sequence similarity of 95% or more across the two collection sites using two DNA metabarcoding markers (ITS1 and ITS2) were assigned to putative fungal genera in 59 families, 27 orders, and 3 phyla. The number of putative fungal genera and the relative abundance of reads for each genus are higher in the tropical rainforest site than in the tropical seasonal forest site. Most of the identified genera are ligninolytic and cellulolytic and/or hemicellulolytic Basidiomycota (Agaricomycetes) and Ascomycota (Sordariomycetes), but “sugar fungi” and fungi associated with plants and detritivorous insects were also detected. This is the first study using NGS as a rapid and large-scale useful strategy to uncover the diversity of wood-decaying fungi in tropical forests.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofPhytotaxapt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectFungal metabarcodingpt_BR
dc.subjectRainforestpt_BR
dc.subjectSeasonal tropical forestpt_BR
dc.subjectLignocellulosic residuespt_BR
dc.subject.otherMetagenômicapt_BR
dc.subject.otherMadeira-microbiologiapt_BR
dc.subject.otherFloresta Úmidapt_BR
dc.subject.otherFlorestas tropicaispt_BR
dc.titleUsing Next-Generation Sequencing (NGS) to uncover diversity of wood-decaying fungi in neotropical atlantic forestspt_BR
dc.title.alternativeUsando Sequenciamento de Nova Geração (NGS) para descobrir a diversidade de fungos decompositores de madeira em florestas atlânticas neotropicaispt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.295.1.1pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-3167-6308pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9038-4631pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0602-7867pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7446-1342pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7692-6243pt_BR
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USING Next-Generation Sequencing (NGS) TO UNCOVER DIVERSITY OF WOOD-DECAYING FUNGI IN NEOTROPICAL ATLANTIC FORESTS.pdf7.21 MBAdobe PDFView/Open
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