Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/55292
Type: Artigo de Periódico
Title: IgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosis
Authors: Laura Néspoli Nassar Pansinide Jesus
Daniel Vitor Vasconcelos-santos
José Nélio Januário
Andréa Teixeira-carvalho
Ricardo Wagner Almeida Vitor
Eloísa A.v. Ferro
José Roberto Mineo
Lilian Maria Garcia Bahia-oliveira
Olindo Assis Martins-filho
Elenice Moreira Lemos
Aline de Castro Zacche Tonini
Geisa Baptista Barros
Jordana Grazziela a. Coelho-dos-reis
Samantha Ribeiro Béla
Lis Ribeiro do Valle Antonelli
Anderson Silva Machado
Ana Carolina de Aguiar Vasconcelos Carneiro
Gláucia Manzan Queiroz Andrade
Abstract: This study intended to apply the flow cytometric analysis of IgA and IgG reactivity and intracytoplasmic cytokine analysis to understand and decode the clinical aspects of infants with ocular congenital toxoplasmosis. The Toxoplasma gondii-infected infants (TOXO) were subdivided according to their clinical aspects based on the absence (NRL), presence of active (ARL), active/cicatricial (ACRL) or cicatricial retinochoroidal lesions (CRL) and compared to non-infected controls (NI). The reactivity of anti-T. gondii IgG subclasses resembles the clinical aspects of ocular lesions. IgG and IgG1 discriminate infants with cicatricial lesions (ACRL and CRL) from both ARL and NLR. IgG2 and IgG3 are particularly higher in ACRL and CRL as compared to NLR. No differences were ob served when IgG4 reactivity was evaluated. Thus, the results indicated that the reactivity patterns of IgA, IgG and IgG subclasses are able to discriminate ARL, ACRL and CRL from NLR or NI. IgA and IgG subclasses are relevant serological biomarkers with diagnostic and prognostic applicability, respectively. Moreover, IgA and IgG1 wereclosely related to cytokine production by innate/adaptive immunity cells. IgA reactivity was directly associated to TNF-α-derived from neutrophils, monocytes and CD8+ T-cells, while IgG1 was inversely correlated with IFN-γ-producing CD4+ and CD8+ T-cells but positively correlated with IL-10+ B-cells. These findings provide insights on the relationship between the cytokine production by innate/adaptive immunity and the antibody pattern of infants with ocular congenital toxoplasmosis. In addition, the present study supports the use of flow cytometric serology as a potential tool for the diagnosis and monitoring of ocular lesions in T. gondii-infected infants in the clinical setting.
Subject: Toxoplasma
Toxoplasmose Congênita
Imunoglobulina A
Imunoglobulina G
language: eng
metadata.dc.publisher.country: Brasil
Publisher: Universidade Federal de Minas Gerais
Publisher Initials: UFMG
metadata.dc.publisher.department: ICB - DEPARTAMENTO DE PARASITOLOGIA
MED - DEPARTAMENTO DE CLÍNICA MÉDICA
Rights: Acesso Restrito
metadata.dc.identifier.doi: 10.1016/j.jim.2015.11.004
URI: http://hdl.handle.net/1843/55292
Issue Date: 2016
metadata.dc.url.externa: https://www.sciencedirect.com/science/article/pii/S0022175915300600
metadata.dc.relation.ispartof: Journal of Immunological Methods
Appears in Collections:Artigo de Periódico

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