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http://hdl.handle.net/1843/56281| Tipo: | Artigo de Periódico |
| Título: | Targeting Wistar rat as a model for studying benign, premalignant and malignant lesions of the prostate |
| Autor(es): | Gabriel Henrique Campolina Silva Hipácia Werneck Gomes Bruna T. Maria Maria C. Barata María J. Torres Hector Contreras Germán A.B. Mahecha Cleida Aparecida de Oliveira |
| Resumo: | Aims: The purpose of this study was to describe a suitable experimental model for studying aging-related prostate disorders including cancer. Materials and methods: 12-month old Wistarratswere keptincontrol conditions (n=12)or treated(n= 16)for 6 months with Silastic implants filled with testosterone (T) and estradiol (E2). After the experiment period (at 18 months of age), animals were euthanized and the prostate and other organs were harvested, dissected, weighed, and processed for morphological, ultrastructural and molecular analyses. Key findings: We demonstrated that male rats of Wistar strain nicely recapitulate the carcinogenesis process taking place in the aging prostate through the arising of benign, precancerous and malignant lesions, and above all yields a modest incidence of spontaneous PCa (~36%). Moreover, our results highlight that 100% incidence of PCa and precancerous lesions such as prostatic intraepithelial neoplasia and proliferative inflammatory atrophy were achieved in this ratstrain after T+ E2 treatment, without changing the broad spectrum of changes that naturally emerge in the prostate at advanced ages. Such enhancement of precancerous lesions and tumors was linked to a decreased expression of E-cadherin andβ-catenin in parallel with an increase in Vimentin and Ncadherin, hallmark modifications of epithelial-mesenchymal transition. Significance: Our findings provide solid evidence that aged Wistar rats may be an excellent model for studies regarding human prostate biology and related disorders including cancer. |
| Assunto: | Prostata Adenocarcinoma Envelhecimento |
| Idioma: | por |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Instituição: | UFMG |
| Departamento: | ICB - DEPARTAMENTO DE MORFOLOGIA |
| Tipo de Acesso: | Acesso Aberto |
| Identificador DOI: | https://doi.org/10.1016/j.lfs.2019.117149 |
| URI: | http://hdl.handle.net/1843/56281 |
| Data do documento: | 2020 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S002432051931077X |
| metadata.dc.relation.ispartof: | Life Sciences |
| Aparece nas coleções: | Artigo de Periódico |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| Targeting Wistar rat as a model for studying benign, premalignant and malignant lesions of the prostate.pdf | 8.58 MB | Adobe PDF | Visualizar/Abrir |
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