Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/56423
Type: Artigo de Periódico
Title: Reactivation of latent HIV-1 in vitro using an ethanolic extract from Euphorbia umbellata (Euphorbiaceae) latex
Authors: Ana Luiza Chaves Valadão
Paulo Damasco
Gabriel Gonçalves
Rui Manuel Reis
Renato Santana de Aguiar
Cleonice Alves de Melo Bento
Amilcar Tanuri
Paula Pezzuto
Viviane A. Oliveira Silva
Barbara Simonson Gonçalves
Átila Duque Rossi
Rodrigo Delvecchio da Cunha
Antonio Carlos Siani
João Batista de Freitas Tostes
Marcelo Trovó
Abstract: Euphorbia umbellata (E. umbellata) belongs to Euphorbiaceae family, popularly known as Janauba, and its latex contains a combination of phorbol esters with biological activities described to different cellular protein kinase C (PKC) isoforms. Here, we identified deoxi-phorbol esters present in E. umbellata latex alcoholic extract that are able to increase HIV transcription and reactivate virus from latency models. This activity is probably mediated by NF-kB activation followed by nuclear translocation and binding to the HIV LTR promoter. In addition, E. umbellata latex extract induced the production of pro inflammatory cytokines in vitro in human PBMC cultures. This latex extract also activates latent virus in human PBMCs isolated from HIV positive patients as well as latent SIV in non-human primate primary CD4+ T lymphocytes. Together, these results indicate that the phorbol esters present in E. umbellata latex are promising candidate compounds for future clinical trials for shock and kill therapies to promote HIV cure and eradication.
Subject: Euforbiacea
Infecções por HIV
Latex
language: por
metadata.dc.publisher.country: Brasil
Publisher: Universidade Federal de Minas Gerais
Publisher Initials: UFMG
metadata.dc.publisher.department: ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS
Rights: Acesso Aberto
metadata.dc.identifier.doi: https://doi.org/10.1371/journal.pone.0207664
URI: http://hdl.handle.net/1843/56423
Issue Date: 2018
metadata.dc.url.externa: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207664
metadata.dc.relation.ispartof: PLOS ONE
Appears in Collections:Artigo de Periódico



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