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Type:	Artigo de Periódico
Title:	Millipore xmap® luminex (hatmag-68k): an accurate and cost-effective method for evaluating alzheimer's biomarkers in cerebrospinal fluid
Authors:	Erika Oliveirahansen Aloisio Joaquim Freitas Ribeiro Bernardo Mattos Viana Maria Aparecida Camargos Bicalho Natalia Silva Dias Ivonne Carolina Bolaños Burgos Monica Vieira Costa Andréa Teixeira Carvalho Antonio Lucio Teixeira Izabela Guimarães Barbosa Lorena Aline Valu Santos Daniela Valadão Freitas Rosa
Abstract:	Background: Alzheimer’s disease (AD) biomarkers are of great relevance in clinical research, especially after the AT(N) framework. They enable early diagnosis, disease staging and research with new promising drugs, monitoring therapeutic response.However, the high cost and low availability of the most well-known methods limits their use in low and medium-income countries. In this context, Millipore xMap® Luminex may be a cost-effective alternative. In our study, using INNOTEST® as reference, we assess the diagnostic accuracy of Millipore xMap® and propose a cutoff point for AD. Methods: We performed lumbar puncture of seven older individuals with clinically defined AD, 17 with amnestic mild cognitive impairment (aMCI) and 11 without objective cognitive impairment-control group (CG). Cerebrospinal fluid (CSF) biomarkers concentrations for aB42, p-Tau, and t-Tau were measured by INNOTEST® and Millipore xMap®, and then the techniques were compared to assess the diagnostic accuracy of the new test and to define a cutoff. Results: INNOTEST® and Millipore xMap® measurements showed all correlations >0.8 for the same biomarker, except for t-Tau that was 0.66. Millipore xMap® measurements showed a robust accuracy for all biomarkers, with AUC higher than 0.808 (t-Tau), and the best for Aβ42 (AUC = 0.952). The most accurate cutoffs were found at 1012.98 pg/ml (Aβ42), 64.54 pg/ml (p-tau), 3251.81 pg/ml (t-tau), 3.370 (t-Tau/Aβ42), and 0.059 (p-Tau/Aβ42). Conclusion: Given its good accuracy and cost-effectiveness, Milliplex xMap® tests seems a reliable and promising tool, especially for low and middle-income countries.
Subject:	Doença de Alzheimer Proteínas tau
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICX - DEPARTAMENTO DE ESTATÍSTICA MED - DEPARTAMENTO DE CLÍNICA MÉDICA MED - DEPARTAMENTO DE SAÚDE MENTAL
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.3389/fpsyt.2021.716686
URI:	http://hdl.handle.net/1843/56602
Issue Date:	31-Aug-2021
metadata.dc.url.externa:	https://www.frontiersin.org/articles/10.3389/fpsyt.2021.716686/full
metadata.dc.relation.ispartof:	Frontiers in Psychiatry
Appears in Collections:	Artigo de Periódico

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