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Type:	Artigo de Periódico
Title:	Subgingival microbiota dysbiosis in systemic lupus erythematosus: association with periodontal status
Authors:	Jôice Dias Corrêa Tarcília Aparecida da Silva Débora Cerqueira Calderaro Gilda Aparecida Ferreira Santuza Maria Souza Mendonça Gabriel R. Fernandes E. Xiao Antônio Lúcio Teixeira Eugene J. Leys Dana T. Graves
Abstract:	Background: Periodontitis results from the interaction between a subgingival biofilm and host immune response.Changes in biofilm composition are thought to disrupt homeostasis between the host and subgingival bacteria resulting in periodontal damage. Chronic systemic inflammatory disorders have been shown to affect the subgingival microbiota and clinical periodontal status. However, this relationship has not been examined in subjects with systemic lupus erythematosus (SLE). The objective of our study was to investigate the influence of SLE on the subgingival microbiota and its connection with periodontal disease and SLE activity.Methods: We evaluated 52 patients with SLE compared to 52 subjects without SLE (control group). Subjects were classified as without periodontitis and with periodontitis. Oral microbiota composition was assessed by amplifying the V4 region of 16S rRNA gene from subgingival dental plaque DNA extracts. These amplicons were examined by Illumina MiSeq sequencing.Results: SLE patients exhibited higher prevalence of periodontitis which occurred at a younger age compared to subjects of the control group. More severe forms of periodontitis were found in SLE subjects that had higher bacterial loads and decreased microbial diversity. Bacterial species frequently detected in periodontal disease were observed in higher proportions in SLE patients, even in periodontal healthy sites such as Fretibacterium, Prevotella nigrescens, and Selenomonas. Changes in the oral microbiota were linked to increased local inflammation, as demonstrated by higher concentrations of IL-6, IL-17, and IL-33 in SLE patients with periodontitis. Conclusions: SLE is associated with differences in the composition of the microbiota, independently of periodontal status.
Subject:	Lupus Periodontite Citocinas Placa Dentária Sequenciamento de Nucleotídeos em Larga Escala
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	MED - DEPARTAMENTO DE APARELHO LOCOMOTOR MED - DEPARTAMENTO DE CLÍNICA MÉDICA
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.1186/s40168-017-0252-z
URI:	http://hdl.handle.net/1843/56795
Issue Date:	2017
metadata.dc.url.externa:	https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0252-z
metadata.dc.relation.ispartof:	Microbiome
Appears in Collections:	Artigo de Periódico

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