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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/58304
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dc.creatorPedro Rodrigues de Almeida IIIpt_BR
dc.creatorAndré Melro Muradpt_BR
dc.creatorLuciano Paulino da Silvapt_BR
dc.creatorElibio Leopoldo Rech Filhopt_BR
dc.creatorElmo Salomão Alvespt_BR
dc.date.accessioned2023-08-28T18:36:53Z-
dc.date.available2023-08-28T18:36:53Z-
dc.date.issued2020-
dc.citation.volume10pt_BR
dc.citation.issue12pt_BR
dc.citation.spage1pt_BR
dc.citation.epage9pt_BR
dc.identifier.doihttps://doi.org/10.3390/bios10120206pt_BR
dc.identifier.issn2079-6374pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/58304-
dc.description.resumoWe present a graphene-based biosensor selective to recombinant cyanovirin-N (rCV-N),an antiviral protein that has proven to be an effective microbicide to inhibit HIV replication. We modified the graphene monolayer devices with 1-pyrenebutanoic acid succinimidyl ester, which interacts with both graphene and the primary and secondary amines of antibodies. By monitoring the change in the electrical resistance of the device, we were able to detect rCV-N in solutions in the range of 0.01 to 10 ng/mL, and found that the detection limit was 0.45 pg/mL, which is much smaller than that obtained with currently available techniques. This is important for applications of this microbicide against HIV, since it may be produced at a large scale from soya bean seeds processed using the available industrial processing technologies. The sensor showed high sensitivity, selectivity, and reproducibility.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE FÍSICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBiosensors-
dc.rightsAcesso Abertopt_BR
dc.subjectGraphene biosensorpt_BR
dc.subjectElectrochemical sensorpt_BR
dc.subjectGenetically modified soybeanpt_BR
dc.subjectRecombinant cyanovirin-Npt_BR
dc.subject.otherGrafenopt_BR
dc.subject.otherSensorespt_BR
dc.titleDevelopment of a graphene-based biosensor for detecting recombinant cyanovirin-Npt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.mdpi.com/2079-6374/10/12/206pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8514-5265pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9075-7725pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8588-0673pt_BR
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