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Type: Artigo de Periódico
Title: Integrated proteomics, phosphoproteomics and metabolomics analyses reveal similarities among giant cell granulomas of the jaws with different genetic mutations
Authors: Jéssica Gardone Vitório
Liséte Celina Lange
Lucilaine Valéria de Souza Santos
Martin Røssel Larsen
Carolina Cavalieri Gomes
Ricardo Santiago Gomez
Filipe Fideles Duarte-Andrade
Thais dos Santos Fontes Pereira
Marcella Nunes de Melo Braga
Gisele André Baptista Canuto
Adriana Nori de Macedo
Yuri Abner Rocha Lebron
Victor Rezende Moreira
Liza Figueiredo Felicori
Abstract: Background: Giant cell granuloma of the jaws are benign osteolytic lesions of the jaws. These lesions are genetically characterized by mutually exclusive somatic mutations at TRPV4, KRAS, and FGFR1, and a fourth molecular subgroup which is wild-type for the three mutations. Irrespective of the molecular background, giant cell granulomas show MAPK/ERK activation. However, it remains unclear if these mutations lead to differences in their molecular signaling in giant cell granulomas. Methods: Metabolomics, proteomics, and phosphoproteomics analyses were carried out in formalin-fixed paraffin-embedded samples of giant cell granuloma of the jaws. The study cohort consisted of five lesions harboring mutations in FGFR1, six in KRAS, five in TRPV4, and five that were wild-type for these mutations. Results: Lesions harboring KRAS or FGFR1 mutations showed overall similar proteomics and metabolomics profiles. In all four groups, metabolic pathways showed similarity in apoptosis, cell signaling, gene expression, cell differentiation, and erythrocyte activity. Lesions harboring TRPV4 mutations showed a greater number of enriched pathways related to tissue architecture. On the other hand, the wild-type group presented increased number of enriched pathways related to protein metabolism compared to the other groups. Conclusion: Despite some minor differences, our results revealed an overall similar molecular profile among the groups with different mutational profile at the metabolic, proteic, and phosphopeptidic levels.
Subject: Biologia molecular
Células - Patogênese
Preparação de amostra (Química)
language: eng Brasil
Publisher: Universidade Federal de Minas Gerais
Publisher Initials: UFMG
metadata.dc.publisher.department: ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
Rights: Acesso Restrito
Issue Date: 2022
metadata.dc.relation.ispartof: Journal of Oral Pathology & Medicine
Appears in Collections:Artigo de Periódico

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