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http://hdl.handle.net/1843/60283| Type: | Artigo de Periódico |
| Title: | Staphylococcus aureus-Cure-Associated Antigens Elicit Type 3 Immune Memory T Cells |
| Authors: | Kamila R. Santos Angélica Rosa Faria Hélida Monteiro de Andrade Mônica M. O. P. Cerqueira Magnus Gidlund Hiro Goto Alice Maria M. P. Della Libera Fernando N. Souza Eduardo M. Ramos-Sanchez Camila F. Batista Luiza C. Reis Wesley L. Fotoran Marcos B. Heinemann Adriano F. Cunha Mussya C. Rocha |
| Abstract: | Background: Staphylococcus aureus is one of the most frequently major mastitis pathogens that cause clinical and subclinical mastitis worldwide. Current antimicrobial treatments are usually ineffective, and the commercially available vaccines lack proven effectiveness. The immunological response elicited by the recombinant S. aureus-cure-associated proteins phosphoglycerate kinase (PGK), enolase (ENO), and elongation factor-G (EF-G) in combination with the granulocyte-macrophage colony-stimulating factor (GM-CSF) DNA vaccination was studied in this work. Methods: Here, twenty-three C57BL/6 mice were divided into four groups and vaccinated with: G1: none (control); G2: GM-CSF DNA plasmid DNA vaccine; G3: the combination of EF-G+ENO+PGK; and G4: the combinations of EF-G+ENO+PGK proteins plus GM-CSF plasmid DNA vaccine. After 44 days, spleen cells were collected for immunophenotyping and lymphocyte proliferation evaluation by flow cytometry upon S. aureus stimulus. Results: Immunization with the three S. aureus recombinant proteins alone resulted in a higher percentage of IL-17A+ cells among CD8+ T central memory cells, as well as the highest intensity of IL-17A production by overall lymphocytes indicating that the contribution of the combined lymphocyte populations is crucial to sustaining a type 3 cell immunity environment. Conclusion: The immunization with three S. aureus-cure-associated recombinant proteins triggered type 3 immunity, which is a highly interesting path to pursue an effective bovine S. aureus mastitis vaccine. |
| Subject: | Vacinação Células T. Infecções Laticínios |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS FARMACIA - FACULDADE DE FARMACIA ICB - DEPARTAMENTO DE PARASITOLOGIA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.3390/antibiotics11121831 |
| URI: | http://hdl.handle.net/1843/60283 |
| Issue Date: | 2-Oct-2022 |
| metadata.dc.url.externa: | https://www.mdpi.com/2079-6382/11/12/1831 |
| metadata.dc.relation.ispartof: | Antibiotics |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Staphylococcus aureus-Cure-Associated Antigens Elicit Type 3 Immune Memory T Cells.pdf | 492.7 kB | Adobe PDF | View/Open |
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