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http://hdl.handle.net/1843/60527| Type: | Artigo de Periódico |
| Title: | Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion |
| Authors: | Fernanda Ludolf Fernanda Fonseca Ramos Eduardo Antonio Ferraz Coelho Flávia F. Bagno João A. Oliveira-da-Silva Thiago A. R. Reis Myron Christodoulides Paula F. Vassallo Cecilia G. Ravetti Vandack Nobre Flavio G. da Fonseca |
| Abstract: | Serum-based ELISA (enzyme-linked immunosorbent assay) has been widely used to detect anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies. However, to date, no study has investigated patient urine as a biological sample to detect SARS-CoV-2 virus-specific antibodies. An in-house urine-based ELISA was developed using recombinant SARS-CoV-2 nucleocapsid protein. The presence of SARS-CoV-2 antibodies in urine was established, with 94% sensitivity and 100% specificity for the detection of anti–SARS-CoV-2 antibodies with the urine-based ELISA and 88% sensitivity and 100% specificity with a paired serum-based ELISA. The urine-based ELISA that detects anti–SARS-CoV-2 antibodies is a noninvasive method with potential application as a facile COVID-19 immunodiagnostic platform, which can be used to report the extent of exposure at the population level and/or to assess the risk of infection at the individual level. |
| Subject: | Imunologia Biotecnologia COVID-19 Urina Diagnóstico |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | MED - DEPARTAMENTO DE CLÍNICA MÉDICA MED - DEPARTAMENTO DE MEDICINA PREVENTIVA SOCIAL MEDICINA - FACULDADE DE MEDICINA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1126/sciadv.abn7424 |
| URI: | http://hdl.handle.net/1843/60527 |
| Issue Date: | 13-May-2022 |
| metadata.dc.url.externa: | https://www.science.org/doi/10.1126/sciadv.abn7424 |
| metadata.dc.relation.ispartof: | Science Advances |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Detecting anti–SARS-CoV-2 antibodies in urine samples_ A noninvasive and sensitive way to assay COVID-19 immune conversion.pdf | 697.76 kB | Adobe PDF | View/Open |
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