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http://hdl.handle.net/1843/60574| Type: | Artigo de Periódico |
| Title: | Unveiling metabolic changes in marsupialized odontogenic keratocyst: a pilot study |
| Authors: | Flávia Leite Lima Victor Rezende Moreira Lucilaine Valéria de Souza Santos Liséte Celina Lange Adriana Nori de Macedo Marina Gonçalves Diniz Carolina Cavaliéri Gomes Wagner Henriques de Castro Gisele André Baptista Canuto Ricardo Santiago Gomez Victor Coutinho Bastos Jéssica Gardone Vitório Filipe Fideles Duarte-Andrade Thaís dos Santos Fontes Pereira Roberta Rayra Martins Chaves Aline Fernanda Cruz Júlio César Tanos de Lacerda Yuri Abner Rocha Lebron |
| Abstract: | Objective: We aimed to assess which metabolic pathways would be implicated in the phenotypic changes of the epithelial lining of odontogenic keratocyst after marsupialization, comparing pre- and post-marsupialized lesions with adjacent oral mucosa. Materials and Methods: Eighteen formalin-fixed and paraffin-embedded tissues from six subjects were divided into three paired groups: odontogenic keratocyst pre- (n = 6) and post-marsupialization (n = 6), and adjacent oral mucosa (n = 6). The metabolic pathways found in these groups were obtained by high-performance liquid chromatography-mass spectrometry-based untargeted metabolomics performed. Results: Through putative metabolite annotation followed by pathway enrichment and predictive analysis with automated algorithms (Mummichog and Gene Set Enrichment Analysis), we found differences in many cellular processes that may be involved in inflammation, oxidative stress response, keratinocyte-basal membrane attachment, differentiation, and proliferation functions, all relevant to odontogenic keratocyst pathobiology and the phenotype acquired after marsupialization.Conclusion: Our study was able to identify several metabolic pathways potentially involved in the metaplastic changes induced by marsupialization of odontogenic keratocysts. An improved comprehension of this process could pave the way for the development of targeted therapies. |
| Subject: | Tumores odontogênicos Boca - Tumores Mucosa bucal Cromatografia líquida de alta eficiência Espectrometria de massa |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE MORFOLOGIA ICB - DEPARTAMENTO DE PATOLOGIA ICX - DEPARTAMENTO DE QUÍMICA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1111/odi.13913 |
| URI: | http://hdl.handle.net/1843/60574 |
| Issue Date: | 2022 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/full/10.1111/odi.13913 |
| metadata.dc.relation.ispartof: | Oral Diseases |
| Appears in Collections: | Artigo de Periódico |
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