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Type:	Artigo de Periódico
Title:	MutSα expression predicts a lower disease-free survival in malignant salivary gland tumors: an immunohistochemical study
Authors:	Gleyson Kleber do Amaral Silva Vivian Petersen Wagner Laryssa Moura Dias Bruno Augusto Linhares Almeida Mariz Felipe Paiva Fonseca Ana Lúcia Carrinho Ayroza Rangel Virgilio Gonzales Zanella Rogério Moraes Castilho Manoela Domingues Martins Pablo Agustin Vargas
Abstract:	Background Appropriate DNA replication is vital to maintain cell integrity at the genomic level. Malfunction on DNA repair mechanisms can have implications related to tumor behavior. Our aim was to evaluate the expression of key complexes of the DNA mismatch-repair system MutSα (hMSH2-hMSH6) and MutSβ (hMSH2-hMSH3) in a panel comprising the most common benign and malignant salivary gland tumors (SGT), and to determine their association with disease-free survival. Material and Methods Ten cases of normal salivary gland (NSG) and 92 of SGT (54 benign and 38 malignant) were retrieved. Immunohistochemistry was performed for hMSH2, hMSH3, hMSH6. Scanned slides were digitally analyzed based on the percentage of positive cells with nuclear staining. Cases were further classified in MutSαhigh and MutSβhigh based on hMSH2-hMSH6 and hMSH3-hMSH6 expression, respectively. Results hMSH3 expression was lower in malignant SGT compared to NSG and benign cases. Adenoid cystic carcinoma (ACC) cases with perineural invasion presented a lower percentage of hMSH3 positive cells. hMSH6 was downregulated in both benign and malignant SGT compared to NSG. Malignant SGT cases with MutSαhigh expression had lower disease-free survival compared to MutSαlow cases. A 10.26-fold increased risk of presenting local recurrence was observed. Conclusions Our findings suggest that a lack of hMSH3 protein function is associated with a more aggressive phenotype (malignancy and perineural invasion) and that MutSα overexpression predicts a poor clinical outcome in malignant SGT.
Subject:	Tumores Biomarcadores Glândulas Salivares Reparo do DNA Neoplasias
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA FAO - DEPARTAMENTO DE ODONTOPEDIATRIA E ORTODONTIA
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.4317%2Fmedoral.25138
URI:	http://hdl.handle.net/1843/61014
Issue Date:	20-Feb-2022
metadata.dc.url.externa:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8898574/
metadata.dc.relation.ispartof:	Med Oral Patol Oral Cir Bucal
Appears in Collections:	Artigo de Periódico

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