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http://hdl.handle.net/1843/61249| Type: | Artigo de Periódico |
| Title: | Antibacterial effect of synthetic peptide LyeTxI and LyeTxI/ß-Cyclodextrin association compound against planktonic and multispecies biofilms of periodontal pathogens |
| Authors: | Edison Andrés Cruz Olivo Daniel Santos Maria Elena de Lima Vera Lúcia dos Santos Rubén Dario Sinisterra Millán María Esperanza Cortés Segura |
| Abstract: | Background: Antimicrobial peptides (AMPs) have shown rapid and potent effect against planktonic bacteria. However, control of periodontopathic biofilms is a challenge even for AMPs. Thus, the present study evaluates in vitro antimicrobial activity of synthetic peptide LyeTxI and association compound LyeTxI/β-cyclodextrin (βCD) against multispecies biofilms. Methods: Sensibility to LyeTxI and LyeTxI/βCD was determined for planktonic Gram-negative periodontopathogens. Time-kill kinetic assay was performed at minimum inhibitory concentrations (MICs) in all planktonic strains. Multispecies biofilms were grown on pegs using a biofilm device and studied by scanning electron microscopy at 2, 5, and 10 days. Minimal biofilm eradication concentration (MBEC) was determined for 2- and 4-day multispecies biofilms. Metabolic activity of biofilms was determined by fluorometry study. Results: Biofilms showed reproducible cell density on pegs of the biofilm device. LyeTxI and LyeTxI/βCD were active against all strains tested at concentrations ≤62.5 μg/mL. Kinetic assays showed rapid bactericidal effect of LyeTxI against all periodontopathogens. MBECs of LyeTxI and LyeTxI/βCD against multispecies 2-day biofilms were two-fold higher than MICs of cells shed from biofilms. LyeTxI was able to reduce multispecies 2-day metabolic activity by 90%. Multispecies 4-day biofilms were tolerant to all agents tested. Conclusions: LyeTxI and LyeTxI/βCD are active against periodontopathic bacteria, showing rapid bactericidal effect and may be used to prevent biofilm development. In the future, AMPs could be therapeutic tools for treatment of periodontitis. |
| Subject: | Ciclodextrinas Biofilme Doença periodontal Agentes antibacterianos Periodontite Microscopia eletrônica de varredura Fluorimetria |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE MICROBIOLOGIA ICX - DEPARTAMENTO DE QUÍMICA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1902/jop.2016.160438 |
| URI: | http://hdl.handle.net/1843/61249 |
| Issue Date: | Jun-2017 |
| metadata.dc.url.externa: | https://aap.onlinelibrary.wiley.com/doi/10.1902/jop.2016.160438 |
| metadata.dc.relation.ispartof: | Journal of Periodontology |
| Appears in Collections: | Artigo de Periódico |
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