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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/61294
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dc.creatorPatricia Donado Vaz de Melopt_BR
dc.creatorAdriane Pimenta da Costa-Val Bicalhopt_BR
dc.creatorFabiola de Oliveira Paes-Lemept_BR
dc.creatorEliana Matias de Souzapt_BR
dc.creatorMariah Gois Ceregattipt_BR
dc.creatorMarco Túlio Gomes Campospt_BR
dc.date.accessioned2023-11-22T22:14:23Z-
dc.date.available2023-11-22T22:14:23Z-
dc.date.issued2022-06-30-
dc.citation.volume15pt_BR
dc.citation.issue6pt_BR
dc.citation.spage1595pt_BR
dc.citation.epage1600pt_BR
dc.identifier.doiwww.doi.org/10.14202/vetworld.2022.1595-1600pt_BR
dc.identifier.issn0972-8988pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/61294-
dc.description.resumoBackground and Aim: Acute kidney injury (AKI) is associated with a grave prognosis. A clinical assessment of kidney function can be performed based on the glomerular filtration rate (GFR). Cystatin C (CysC) can indicate the GFR or kidney function and its measurement is currently performed using immunological methods such as nephelometry, immunoturbidimetry, and enzyme-linked immunosorbent assays in human medicine. However, these techniques are not specific for use in veterinary medicine. This study aimed to validate an immunoturbidimetric assay for serum CysC (sCy) in dogs, determine the sCy reference intervals for healthy dogs, evaluate sCy stability in serum samples, and compare sCy with serum creatinine (sCr) in healthy dogs and dogs with AKI. Materials and Methods: Forty-three dogs were divided into a control group (n = 19) and an AKI group (n = 24). An immunoturbidimetric method including commercially available human CysC calibrated with canine CysC was used to evaluate canine serum samples. Results: An average recovery of 97% was observed for canine serum samples. The reference interval for CysC in healthy dogs was 0.57–1.29 mg/L. The sCy concentration in dogs with AKI was significantly higher (2.82 ± 1.46 mg/L) than in healthy dogs (0.93 ± 0.18 mg/L). Statistical analysis confirmed a strong correlation between sCy and sCr (r = 0.94; p < 0.05) in dogs with AKI. Conclusion: The immunoturbidimetric method of evaluating sCy yielded satisfactory results and can be used for canine samples when a species-specific calibrator is used. Furthermore, sCy is a reliable marker of renal dysfunction in dogs. It is best to store samples for sCy evaluation at temperatures between 4°C and 8°C.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentVET - DEPARTAMENTO DE CLÍNICA E CIRURGIApt_BR
dc.publisher.departmentVETER - ESCOLA DE VETERINARIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofVeterinary Worldpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectCaninept_BR
dc.subjectImmunoturbidimetrypt_BR
dc.subjectKidneypt_BR
dc.subject.otherAnimais domésticospt_BR
dc.subject.otherRinspt_BR
dc.titleCystatin C assay validation using the immunoturbidimetric method to evaluate the renal function of healthy dogs and dogs with acute renal injurypt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttp://www.veterinaryworld.org/Vol.15/June-2022/25.htmlpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4013-0147pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-3527-7942pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6679-8295pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6247-2255pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0692-6587pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8995-9805pt_BR
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