Please use this identifier to cite or link to this item:
http://hdl.handle.net/1843/61294| Type: | Artigo de Periódico |
| Title: | Cystatin C assay validation using the immunoturbidimetric method to evaluate the renal function of healthy dogs and dogs with acute renal injury |
| Authors: | Patricia Donado Vaz de Melo Adriane Pimenta da Costa-Val Bicalho Fabiola de Oliveira Paes-Leme Eliana Matias de Souza Mariah Gois Ceregatti Marco Túlio Gomes Campos |
| Abstract: | Background and Aim: Acute kidney injury (AKI) is associated with a grave prognosis. A clinical assessment of kidney function can be performed based on the glomerular filtration rate (GFR). Cystatin C (CysC) can indicate the GFR or kidney function and its measurement is currently performed using immunological methods such as nephelometry, immunoturbidimetry, and enzyme-linked immunosorbent assays in human medicine. However, these techniques are not specific for use in veterinary medicine. This study aimed to validate an immunoturbidimetric assay for serum CysC (sCy) in dogs, determine the sCy reference intervals for healthy dogs, evaluate sCy stability in serum samples, and compare sCy with serum creatinine (sCr) in healthy dogs and dogs with AKI. Materials and Methods: Forty-three dogs were divided into a control group (n = 19) and an AKI group (n = 24). An immunoturbidimetric method including commercially available human CysC calibrated with canine CysC was used to evaluate canine serum samples. Results: An average recovery of 97% was observed for canine serum samples. The reference interval for CysC in healthy dogs was 0.57–1.29 mg/L. The sCy concentration in dogs with AKI was significantly higher (2.82 ± 1.46 mg/L) than in healthy dogs (0.93 ± 0.18 mg/L). Statistical analysis confirmed a strong correlation between sCy and sCr (r = 0.94; p < 0.05) in dogs with AKI. Conclusion: The immunoturbidimetric method of evaluating sCy yielded satisfactory results and can be used for canine samples when a species-specific calibrator is used. Furthermore, sCy is a reliable marker of renal dysfunction in dogs. It is best to store samples for sCy evaluation at temperatures between 4°C and 8°C. |
| Subject: | Animais domésticos Rins |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | VET - DEPARTAMENTO DE CLÍNICA E CIRURGIA VETER - ESCOLA DE VETERINARIA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | www.doi.org/10.14202/vetworld.2022.1595-1600 |
| URI: | http://hdl.handle.net/1843/61294 |
| Issue Date: | 30-Jun-2022 |
| metadata.dc.url.externa: | http://www.veterinaryworld.org/Vol.15/June-2022/25.html |
| metadata.dc.relation.ispartof: | Veterinary World |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Cystatin C assay validation using the immunoturbidimetric method to evaluate the renal function of healthy dogs and dogs with acute renal injury.pdf | 235.39 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.