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Tipo:	Artigo de Periódico
Título:	Diagnosis of visceral and cutaneous leishmaniasis using loop-mediated isothermal amplification (LAMP) protocols: a systematic review and meta-analysis
Autor(es):	Astrid Christine Erber Peter Julian Sandler Daniel Moreira de Avelar Ines Swoboda Glaucia Fernandes Cota Julia Walochnik
Resumen:	Sensitive, reliable and fast diagnostic tools that are applicable in low-resource settings, at the point of care (PoC), are seen as crucial in the fight against visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). Addressing the need for a PoC test, several diagnostic tests, including serological and molecular methods, have been developed and evaluated in the past. One promising molecular method, already implemented for diagnosis of a range of diseases, is the loop-mediated isothermal amplification (LAMP) protocol. In this systematic review and meta-analysis, using a comprehensive search strategy, we focus on studies evaluating the performance of LAMP for the diagnosis of leishmaniasis in humans and other mammals such as dogs, compared with microscopy and/or any other molecular diagnostic method. A meta-analysis, pooling sensitivity and specificity rates and calculating areas under the curve (AUCs) in summary receiver operating characteristic (SROC) plots, was conducted on datasets extracted from studies, grouped by clinical condition and sample type. We found high sensitivity and specificity for LAMP when compared with microscopy and PCR using blood samples, with pooled estimate values of > 90% for all subgroups, corresponding to calculated AUC values > 0.96, except for LAMP compared to microscopy for diagnosis of CL. However, only a limited number of studies were truly comparable. Most of the observed heterogeneity is likely based on true differences between the studies rather than sampling error only. Due to simple readout methods and low laboratory equipment requirements for sample preparation compared to other molecular methods, LAMP is a promising candidate for a molecular (near-)PoC diagnostic method for VL and CL.
Asunto:	Leishmaniose Testes de Diagnóstico Rápido
Idioma:	eng
País:	Brasil
Editor:	Universidade Federal de Minas Gerais
Sigla da Institución:	UFMG
Departamento:	HCL - HOSPITAL DAS CLINICAS
Tipo de acceso:	Acesso Aberto
Identificador DOI:	https://doi.org/10.1186/s13071-021-05133-2
URI:	http://hdl.handle.net/1843/61857
Fecha del documento:	24-ene-2022
metadata.dc.url.externa:	https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-021-05133-2
metadata.dc.relation.ispartof:	Parasites & Vectors
Aparece en las colecciones:	Artigo de Periódico

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