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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/61857
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dc.creatorAstrid Christine Erberpt_BR
dc.creatorPeter Julian Sandlerpt_BR
dc.creatorDaniel Moreira de Avelarpt_BR
dc.creatorInes Swobodapt_BR
dc.creatorGlaucia Fernandes Cotapt_BR
dc.creatorJulia Walochnikpt_BR
dc.date.accessioned2023-12-07T21:39:32Z-
dc.date.available2023-12-07T21:39:32Z-
dc.date.issued2022-01-24-
dc.citation.volume15pt_BR
dc.citation.spage1pt_BR
dc.citation.epage16pt_BR
dc.identifier.doihttps://doi.org/10.1186/s13071-021-05133-2pt_BR
dc.identifier.issn1756-3305pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/61857-
dc.description.resumoSensitive, reliable and fast diagnostic tools that are applicable in low-resource settings, at the point of care (PoC), are seen as crucial in the fight against visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). Addressing the need for a PoC test, several diagnostic tests, including serological and molecular methods, have been developed and evaluated in the past. One promising molecular method, already implemented for diagnosis of a range of diseases, is the loop-mediated isothermal amplification (LAMP) protocol. In this systematic review and meta-analysis, using a comprehensive search strategy, we focus on studies evaluating the performance of LAMP for the diagnosis of leishmaniasis in humans and other mammals such as dogs, compared with microscopy and/or any other molecular diagnostic method. A meta-analysis, pooling sensitivity and specificity rates and calculating areas under the curve (AUCs) in summary receiver operating characteristic (SROC) plots, was conducted on datasets extracted from studies, grouped by clinical condition and sample type. We found high sensitivity and specificity for LAMP when compared with microscopy and PCR using blood samples, with pooled estimate values of > 90% for all subgroups, corresponding to calculated AUC values > 0.96, except for LAMP compared to microscopy for diagnosis of CL. However, only a limited number of studies were truly comparable. Most of the observed heterogeneity is likely based on true differences between the studies rather than sampling error only. Due to simple readout methods and low laboratory equipment requirements for sample preparation compared to other molecular methods, LAMP is a promising candidate for a molecular (near-)PoC diagnostic method for VL and CL.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentHCL - HOSPITAL DAS CLINICASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofParasites & Vectorspt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectLeishmaniasispt_BR
dc.subjectCutaneous leishmaniasispt_BR
dc.subjectVisceral leishmaniasispt_BR
dc.subjectLoop-mediated isothermal amplificationpt_BR
dc.subjectIn vitro diagnosticspt_BR
dc.subjectNeglected tropical diseasept_BR
dc.subjectMeta-analysispt_BR
dc.subject.otherLeishmaniosept_BR
dc.subject.otherTestes de Diagnóstico Rápidopt_BR
dc.titleDiagnosis of visceral and cutaneous leishmaniasis using loop-mediated isothermal amplification (LAMP) protocols: a systematic review and meta-analysispt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-021-05133-2pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0538-7403pt_BR
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