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Type:	Artigo de Periódico
Title:	Oral formulation of angiotensin-(1-7) promotes therapeutic actions in a model of eosinophilic and neutrophilic asthma
Authors:	Giselle Santos Magalhães Juliana Fabiana Gregório Arthur Tonani Pereira Cançado Ribeiro Isis Felippe Baroni Ana Victoria de Oliveira Vasconcellos Gabriela Pansanato Nakashima Isabel Fusaro Aguiar Oliveira Natália Alves de Matos Thalles de Freitas Castro Frank Silva Bezerra Rubén Dario Sinisterra Millán Vanessa Pinho Mauro Martins Teixeira Robson Augusto Souza Santos Maria da Glória Rodrigues-Machado Maria José Campagnole dos Santos
Abstract:	The presence of eosinophils and neutrophils in the lungs of asthmatic patients is associated with the severity of the disease and resistance to corticosteroids. Thus, defective resolution of eosinophilic and neutrophilic inflammation is importantly related to exacerbation of asthma. In this study, we investigated a therapeutic action of angiotensin-(1-7) (Ang-(1-7)) in a model of asthma induced by ovalbumin (OVA) and lipopolysaccharide (LPS). Balb-c mice were sensitized and challenged with OVA. Twenty-three hours after the last OVA challenge, experimental groups received LPS, and 1 h and 7 h later, mice were treated with oral formulation of Ang-(1-7). On the next day, 45 h after the last challenge with OVA, mice were subjected to a test of motor and exploratory behavior; 3 h later, lung function was evaluated, and bronchoalveolar lavage fluid (BALF) and lungs were collected. Motor and exploratory activities were lower in OVA + LPS-challenged mice. Treatment with Ang-(1-7) improved these behaviors, normalized lung function, and reduced eosinophil, neutrophil, myeloperoxidase (MPO), eosinophilic peroxidase (EPO), and ERK1/2 phosphorylation (p-ERK1/2) in the lungs. In addition, Ang-(1-7) decreased the deposition of mucus and extracellular matrix in the airways. These results extended those of previous studies by demonstrating that oral administration of Ang-(1-7) at the peak of pulmonary inflammation can be valuable for the treatment of neutrophil- and eosinophil-mediated asthma. Therefore, these findings potentially provide a new drug to reverse the natural history of the disease, unlike the current standards of care that manage the disease symptoms at best.
Subject:	Farmacologia pulmonar Angiotensina Asma Eosinófilos Neutrófilos Aparelho respiratório -- Doenças
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE FARMACOLOGIA ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA ICB - DEPARTAMENTO DE MORFOLOGIA ICX - DEPARTAMENTO DE QUÍMICA
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.3389/fphar.2021.557962
URI:	http://hdl.handle.net/1843/61924
Issue Date:	2021
metadata.dc.url.externa:	https://www.frontiersin.org/articles/10.3389/fphar.2021.557962/full
metadata.dc.relation.ispartof:	Frontiers in Pharmacology
Appears in Collections:	Artigo de Periódico

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