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http://hdl.handle.net/1843/62980| Type: | Artigo de Periódico |
| Title: | Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil |
| Authors: | Claudio Joséaugusto Wania da Silva Carvalho Isabela Neves de Almeida Lida Jouca de Assis Figueiredo Nayane g. t. Dantas Philip n Suffys Silvana Spindola de Miranda |
| Abstract: | DNA genotyping of Mycobacterium tuberculosis has been widely applied in the understanding of disease transmission in many countries. The purpose of this study was to genotype the strains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais, as well as to compare the similarity, discriminatory power, and agreement of the clusters between the IS6110 Restriction Fragment Length Polymorfism (RFLP) and 12 loci Variable Number Tandem Repeat – Mycobacterial Interspersed Repetitive Units (MIRU-VNTR) tech niques. It was observed that 32% (66/204) of the isolated strains in the RFLP-IS6110 and 50.9% (104/204) of the isolated strains in the MIRU-VNTR presented a similarity of equal to or above 85%. The RFLP-IS6110 and MIRU-VNTR proved to contain a high discriminatory power. The similarity index resulting from the RFLP showed no recent transmission. Good agreement was observed between the techniques when clusters were detected; however,the best epidemiological relationship was found when using the RFLP-IS6110. |
| Subject: | Mycobacterium tuberculosis Polymorphism, Restriction Fragment Length |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAR - DEPARTAMENTO DE FARMÁCIA SOCIAL |
| Rights: | Acesso Aberto |
| URI: | http://hdl.handle.net/1843/62980 |
| Issue Date: | 2017 |
| metadata.dc.url.externa: | https://doi.org/10.1016/j.bjm.2017.04.015 |
| metadata.dc.relation.ispartof: | Brazilian Journal of Microbiology |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Comparative study of RFLP-IS6110 and MIRU-VNTR pdfa.pdf | 781.24 kB | Adobe PDF | View/Open |
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