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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/63266
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dc.creatorMaristela Oliveiralarapt_BR
dc.creatorThabata Coaglio Lucaspt_BR
dc.creatorEvanguedes Kalapothakispt_BR
dc.creatorRonaldo Luis Thomasinipt_BR
dc.creatorCarla Jorge Machadopt_BR
dc.date.accessioned2024-01-23T19:47:50Z-
dc.date.available2024-01-23T19:47:50Z-
dc.date.issued2018-
dc.citation.volume51pt_BR
dc.citation.issue4pt_BR
dc.citation.spage528pt_BR
dc.citation.epage532pt_BR
dc.identifier.doi10.1590/0037-8682-0352-2017pt_BR
dc.identifier.issn16789849pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/63266-
dc.description.resumoIntroduction: Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. Methods: This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. Results: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. Conclusions: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocolspt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE MEDICINA PREVENTIVA SOCIALpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropical-
dc.rightsAcesso Abertopt_BR
dc.subjectPolymerase Chain Reactionpt_BR
dc.subjectDNA, Bacterialpt_BR
dc.subjectDNA extractionpt_BR
dc.subjectNosocomial infectionpt_BR
dc.subject.otherPolymerase Chain Reactionpt_BR
dc.subject.otherDNA, Bacterialpt_BR
dc.subject.otherCross Infectionpt_BR
dc.titleComparison of five methods of extraction of staphylococcus aureus dna for molecular detection by pcrpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://doi.org/10.1590/0037-8682-0352-2017pt_BR
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