Please use this identifier to cite or link to this item:
http://hdl.handle.net/1843/63266| Type: | Artigo de Periódico |
| Title: | Comparison of five methods of extraction of staphylococcus aureus dna for molecular detection by pcr |
| Authors: | Maristela Oliveiralara Thabata Coaglio Lucas Evanguedes Kalapothakis Ronaldo Luis Thomasini Carla Jorge Machado |
| Abstract: | Introduction: Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. Methods: This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. Results: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. Conclusions: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols |
| Subject: | Polymerase Chain Reaction DNA, Bacterial Cross Infection |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | MED - DEPARTAMENTO DE MEDICINA PREVENTIVA SOCIAL |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | 10.1590/0037-8682-0352-2017 |
| URI: | http://hdl.handle.net/1843/63266 |
| Issue Date: | 2018 |
| metadata.dc.url.externa: | https://doi.org/10.1590/0037-8682-0352-2017 |
| metadata.dc.relation.ispartof: | Revista da Sociedade Brasileira de Medicina Tropical |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Comparison of five methods of extraction of pdfa.pdf | 589.19 kB | Adobe PDF | View/Open |
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