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http://hdl.handle.net/1843/64884| Type: | Artigo de Periódico |
| Title: | LNO3 and L3 are associated with antiproliferative and pro-apoptotic action in hepatoma cells |
| Authors: | Leonardo Campos Zanelatto Patrícia Benites Gonçalves da Silva Daniele Sartori Sandra Lepri Ângelo de Fátima Mário Sérgio Mantovani |
| Abstract: | The identification of antitumoral substances is the focus of intense biomedical research. Two structural analogues of thalidomide, LNO3 and L3, are two synthetic compounds that might possess such antitumor properties. We evaluated the toxicological effects of these substances, including cytotoxicity, genotoxicity and induction of apoptosis in HTC cells. Additionally, the production of free radicals (nitric oxide and superoxide) was investigated, and the expression of caspases genes 3, 8, and 9 were determined by RT-qPCR. The compounds exhibited cytotoxic effects that resulted in inhibited cell proliferation. LNO3 showed to be more effective and toxic than L3 in all assays. LNO3 stimulated the release of NO and superoxide, which was accompanied by the formation of peroxynitrite. Apoptosis was induced in a dose-dependent manner by both compounds; however, the expression of caspases 3, 8 and 9 was unchanged. These results suggested that L3 and LNO3 possess antiproliferative and pro-apoptotic effects in HTC cells. Additionally, although they exhibited cytotoxicity, L3 and LNO3 might be useful coadjuvants in tumor treatment studies. |
| Subject: | Bioquímica Química Óxido nítrico Reações de radicais livres Apoptose Agentes antineoplásicos |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICX - DEPARTAMENTO DE QUÍMICA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1590/1678-4685-GMB-2015-0184 |
| URI: | http://hdl.handle.net/1843/64884 |
| Issue Date: | 2016 |
| metadata.dc.url.externa: | https://www.scielo.br/j/gmb/a/VSCtR4XRPdmK7Ydk8xfwS4Q/ |
| metadata.dc.relation.ispartof: | Genetics and Molecular Biology |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| LNO3 AND L3 Are Associated With Antiproliferative And Pro-Apoptotic Action (1).pdf | 574.63 kB | Adobe PDF | View/Open |
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