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Type:	Artigo de Periódico
Title:	Nearest-neighbour parameters optimized for melting temperature prediction of DNA/RNA hybrids at high and low salt concentrations
Authors:	Vivianne Basilio Barbosa Erik de Oliveira Martins Gerald Weber
Abstract:	Gene editing technologies sparked a renewed interest in the hybridization of DNA/RNA duplexes, yet little improvement on nearest-neighbour parameters was made over the past two decades. For low sodium concentration no parameter set was yet calculated. Here, we revised the existing experimental datasets and used an expanded set of sequences from which we recalculated the nearest-neighbour parameters, reducing the average temperature prediction uncertainty to 1.6 °C. Two experimental sets using temperatures extracted via different methods were used with similar results, with the curve-fitting method achieving a slight advantage in prediction quality over other methods. Additionally, we obtained new parameters for low salt with an average uncertainty of 0.98 °C. We also tested several types of salt correction factors and concluded that it is advisable to use those originally developed for RNA/RNA rather than for DNA/DNA.
Subject:	DNA Ácido ribonucleico
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICX - DEPARTAMENTO DE FÍSICA
Rights:	Acesso Restrito
metadata.dc.identifier.doi:	https://doi.org/10.1016/j.bpc.2019.106189
URI:	http://hdl.handle.net/1843/65618
Issue Date:	2019
metadata.dc.url.externa:	https://www.sciencedirect.com/science/article/pii/S0301462219301814?via%3Dihub
metadata.dc.relation.ispartof:	Biophysical Chemistry
Appears in Collections:	Artigo de Periódico

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