Please use this identifier to cite or link to this item:
http://hdl.handle.net/1843/65618
Type: | Artigo de Periódico |
Title: | Nearest-neighbour parameters optimized for melting temperature prediction of DNA/RNA hybrids at high and low salt concentrations |
Authors: | Vivianne Basilio Barbosa Erik de Oliveira Martins Gerald Weber |
Abstract: | Gene editing technologies sparked a renewed interest in the hybridization of DNA/RNA duplexes, yet little improvement on nearest-neighbour parameters was made over the past two decades. For low sodium concentration no parameter set was yet calculated. Here, we revised the existing experimental datasets and used an expanded set of sequences from which we recalculated the nearest-neighbour parameters, reducing the average temperature prediction uncertainty to 1.6 °C. Two experimental sets using temperatures extracted via different methods were used with similar results, with the curve-fitting method achieving a slight advantage in prediction quality over other methods. Additionally, we obtained new parameters for low salt with an average uncertainty of 0.98 °C. We also tested several types of salt correction factors and concluded that it is advisable to use those originally developed for RNA/RNA rather than for DNA/DNA. |
Subject: | DNA Ácido ribonucleico |
language: | eng |
metadata.dc.publisher.country: | Brasil |
Publisher: | Universidade Federal de Minas Gerais |
Publisher Initials: | UFMG |
metadata.dc.publisher.department: | ICX - DEPARTAMENTO DE FÍSICA |
Rights: | Acesso Restrito |
metadata.dc.identifier.doi: | https://doi.org/10.1016/j.bpc.2019.106189 |
URI: | http://hdl.handle.net/1843/65618 |
Issue Date: | 2019 |
metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0301462219301814?via%3Dihub |
metadata.dc.relation.ispartof: | Biophysical Chemistry |
Appears in Collections: | Artigo de Periódico |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.