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Type:	Artigo de Periódico
Title:	High-Salt Diet Induces IL-17-Dependent Gut Inflammation and Exacerbates Colitis in Mice
Authors:	Sarah Leao Fiorini de Aguiar Valbert Nascimento Cardoso Patrícia Aparecida Barros Tatiani Uceli Maioli Ana Maria Caetano Faria Mariana Camila Gonçalves Miranda Mauro Andrade Freitas Guimarães Helton Costa Santiago Camila Pereira Queiroz Pricila da Silva Cunha Denise Carmona Cara Giselle Foureaux Anderson José Ferreira
Abstract:	Excess intake of sodium is often associated with high risk for cardiovascular disease. More recently, some studies on the effects of high-salt diets (HSDs) have also demonstrated that they are able to activate Th17 cells and increase severity of autoimmune diseases. The purpose of the present study was to evaluate the effects of a diet supplemented with NaCl in the colonic mucosa at steady state and during inflammation. We showed that consumption of HSD by mice triggered a gut inflammatory reaction associated with IL-23 production, recruitment of neutrophils, and increased frequency of the IL-17-producing type 3 innate lymphoid cells (ILC3) in the colon. Moreover, gut inflammation was not observed in IL-17–/– mice but it was present, although at lower grade, in RAG−/− mice suggesting that the inflammatory effects of HSD was dependent on IL-17 but only partially on Th17 cells. Expression of SGK1, a kinase involved in sodium homeostasis, increased 90 min after ingestion of 50% NaCl solution and decreased 3 weeks after HSD consumption. Colitis induced by oral administration of either dextran sodium sulfate or 2,4,6-trinitrobenzenesulfonic acid was exacerbated by HSD consumption and this effect was associated with increased frequencies of RORγt+ CD4+ T cells and neutrophils in the colon. Therefore, our results demonstrated that consumption of HSD per se triggered a histologically detectable inflammation in the colon and also exacerbated chemically induced models of colitis in mice by a mechanism dependent on IL-17 production most likely by both ILC3 and Th17 cells.
Subject:	Células Th17 Inflamação Interleucina-17 Cloreto de Sódio
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ENF - DEPARTAMENTO DE NUTRIÇÃO ENFERMAGEM - ESCOLA DE ENFERMAGEM FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE MORFOLOGIA
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.3389/fimmu.2017.01969
URI:	http://hdl.handle.net/1843/66191
Issue Date:	14-Jan-2018
metadata.dc.url.externa:	https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2017.01969/full
metadata.dc.relation.ispartof:	Frontiers in Immunology
Appears in Collections:	Artigo de Periódico

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