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http://hdl.handle.net/1843/76813| Type: | Artigo de Periódico |
| Title: | Using an aluminum hydroxide–chitosan matrix increased the vaccine potential and immune response of mice against multi-drug-resistant Acinetobacter baumannii |
| Authors: | Túllio Teixeira Deusdará Mellanie Karoline do Carmo Félix Hélio de Sousa Brito Edson Wagner Silva Cangussu Wellington de Souza Moura Benedito Albuquerque Marcos Gontijo da Silva Gil Rodrigues dos Santos Paula Benevides de Morais Elizângela Farias da Silva Yury Oliveira Chaves Luís André Morais Mariuba Paulo Afonso Nogueira Spartaco Astolfi-Filho Enedina Nogueira de Assunção Sabrina Epiphanio Claudio Romero Farias Marinho Igor Viana Brandi Kelvinson Fernandes Viana Eugênio Eduardo de Oliveira Alex Sander Rodrigues Cangussu |
| Abstract: | Acinetobacter baumannii is a Gram-negative, immobile, aerobic nosocomial opportunistic coccobacillus that causes pneumonia, septicemia, and urinary tract infections in immunosuppressed patients. There are no commercially available alternative antimicrobials, and multi-drug resistance is an urgent concern that requires emergency measures and new therapeutic strategies. This study evaluated a multi-drug-resistant A. baumannii whole-cell vaccine, inactivated and adsorbed on an aluminum hydroxide–chitosan (mAhC) matrix, in an A. baumannii sepsis model in immunosuppressed mice by cyclophosphamide (CY). CY-treated mice were divided into immunized, non-immunized, and adjuvant-inoculated groups. Three vaccine doses were given at 0D, 14D, and 28D, followed by a lethal dose of 4.0 × 108 CFU/mL of A. baumannii. Immunized CY-treated mice underwent a significant humoral response, with the highest IgG levels and a higher survival rate (85%); this differed from the non-immunized CY-treated mice, none of whom survived (p < 0.001), and from the adjuvant group, with 45% survival (p < 0.05). Histological data revealed the evident expansion of white spleen pulp from immunized CY-treated mice, whereas, in non-immunized and adjuvanted CY-treated mice, there was more significant organ tissue damage. Our results confirmed the proof-of-concept of the immune response and vaccine protection in a sepsis model in CY-treated mice, contributing to the advancement of new alternatives for protection against A. baumannii infections. |
| Subject: | Bactérias anaeróbias Acinetobacter Infecções urinarias Imunossupressão Ciclofosfamida |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.3390/vaccines11030669 |
| URI: | http://hdl.handle.net/1843/76813 |
| Issue Date: | 16-Mar-2023 |
| metadata.dc.url.externa: | https://www.mdpi.com/2076-393X/11/3/669 |
| metadata.dc.relation.ispartof: | Vaccines |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Using an Aluminum Hydroxide–Chitosan Matrix Increased the Vaccine Potential and Immune Response of Mice against Multi-Drug-Resistant Acinetobacter baumannii.pdf | 3.12 MB | Adobe PDF | View/Open |
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