Avaliação da estabilidade de agrotóxicos piretroides e anilinopirimidinas em vinho utilizando cromatografia a líquido acoplada a espectrometria de massas sequencial

Abstract

Wine is one of the most consumed alcoholic beverage in Brazil and around the world, and viticulture occupies a prominent place in the socioeconomic and cultural aspects in several countries. With a globalized market and the need to warrant good quality grapes, it is common to use pesticides on grape crops to prevent parasite attacks. The list of pesticides authorized for use in vineyards, as well as their maximum permitted limit, varies between producing countries. Furthermore, there is no specific legislation regarding the limit of contaminating residues in wine in Brazil and the European Union, except in Switzerland, and each year there are variations in the type and quantity of pesticides applied to grapes. Consequently, pesticide residues are frequently found in wine, making it essential to quantify and study their stability in order to assess the risk of consumer exposure. In this context, the present work quantified and evaluated the stability of some representatives of two groups of pesticides - pyrethroids and anilinopyrimidines - in red and white wine, through the development and optimization of an HPLC-MS/MS method. The extraction of these pesticides was performed by liquid-liquid extraction, followed by the addition of salts (salting out), a technique widely used for the extraction of analytes from food matrices; followed by analysis by highperformance liquid chromatography coupled to tandem mass spectrometry, a technique widely used in determining residues and contaminants in food, due to the analysis time, detection limit and sample yield. The results obtained in the stability study demonstrated that pyrethroid pesticides showed a greater reduction in concentration compared to anilinopyrimidine pesticides, and white wine exhibited higher stability profile for anilinopyrimidine pesticides. A significant matrix effect was also observed in the analysis of these pesticides in red and white wine, so that the use of an analytical curve obtained with the solvent acetonitrile is not recommended for quantitative analysis.