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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/81548
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dc.creatorFlávia Fonseca Bagnopt_BR
dc.creatorSarah Aparecida Rodrigues Sergiopt_BR
dc.creatorMaria Marta Figueiredopt_BR
dc.creatorLara Carvalho Godoipt_BR
dc.creatorLuis Adan Flores Andradept_BR
dc.creatorNatália Salazar de Castropt_BR
dc.creatorCamila Pereira Soarespt_BR
dc.creatorAndressa Simões Aguiarpt_BR
dc.creatorFlávia Jacqueline Almeidapt_BR
dc.creatorEdimilson Domingos da Silvapt_BR
dc.creatorAntonio Gomes Pinto Ferreirapt_BR
dc.creatorEdison Luiz Durigonpt_BR
dc.creatorRicardo Tostes Gazzinellipt_BR
dc.creatorSantuza Maria Ribeiro Teixeirapt_BR
dc.creatorAna Paula Salles Moura Fernandespt_BR
dc.creatorFlávio Guimarães da Fonsecapt_BR
dc.date.accessioned2025-04-14T15:41:28Z-
dc.date.available2025-04-14T15:41:28Z-
dc.date.issued2022-08-
dc.citation.volume2pt_BR
dc.citation.issue3pt_BR
dc.citation.spage1pt_BR
dc.citation.epage6pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.jcvp.2022.100101pt_BR
dc.identifier.issn2667-0380pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/81548-
dc.description.resumoThere is a massive demand to identify alternative methods to detect new cases of COVID-19 as well as to investigate the epidemiology of the disease. In many countries, importation of commercial kits poses a significant impact on their testing capacity and increases the costs for the public health system. We have developed an ELISA to detect IgG antibodies against SARS-CoV-2 using a recombinant viral nucleocapsid (rN) protein expressed in E. coli. Using a total of 894 clinical samples we showed that the rN-ELISA was able to detect IgG antibodies against SARS-CoV-2 with high sensitivity (97.5%) and specificity (96.3%) when compared to a commercial antibody test. After three external validation studies, we showed that the test accuracy was higher than 90%. The rN-ELISA IgG kit constitutes a convenient and specific method for the large-scale determination of SARS-CoV-2 antibodies in human sera with high reliability.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of clinical virology pluspt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectSARS-CoV-2pt_BR
dc.subjectCOVID-19pt_BR
dc.subjectDiagnosispt_BR
dc.subjectSerological assaypt_BR
dc.subjectELISApt_BR
dc.subjectPrototypingpt_BR
dc.subject.otherSARS-CoV-2pt_BR
dc.subject.otherCOVID-19pt_BR
dc.subject.otherDiagnósticopt_BR
dc.subject.otherSorologiapt_BR
dc.subject.otherEnsaio de imunoadsorção enzimáticapt_BR
dc.titleDevelopment and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19pt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S2667038022000400?via%3Dihubpt_BR
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