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http://hdl.handle.net/1843/81548| Type: | Artigo de Periódico |
| Title: | Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19 |
| Authors: | Flávia Fonseca Bagno Sarah Aparecida Rodrigues Sergio Maria Marta Figueiredo Lara Carvalho Godoi Luis Adan Flores Andrade Natália Salazar de Castro Camila Pereira Soares Andressa Simões Aguiar Flávia Jacqueline Almeida Edimilson Domingos da Silva Antonio Gomes Pinto Ferreira Edison Luiz Durigon Ricardo Tostes Gazzinelli Santuza Maria Ribeiro Teixeira Ana Paula Salles Moura Fernandes Flávio Guimarães da Fonseca |
| Abstract: | There is a massive demand to identify alternative methods to detect new cases of COVID-19 as well as to investigate the epidemiology of the disease. In many countries, importation of commercial kits poses a significant impact on their testing capacity and increases the costs for the public health system. We have developed an ELISA to detect IgG antibodies against SARS-CoV-2 using a recombinant viral nucleocapsid (rN) protein expressed in E. coli. Using a total of 894 clinical samples we showed that the rN-ELISA was able to detect IgG antibodies against SARS-CoV-2 with high sensitivity (97.5%) and specificity (96.3%) when compared to a commercial antibody test. After three external validation studies, we showed that the test accuracy was higher than 90%. The rN-ELISA IgG kit constitutes a convenient and specific method for the large-scale determination of SARS-CoV-2 antibodies in human sera with high reliability. |
| Subject: | SARS-CoV-2 COVID-19 Diagnóstico Sorologia Ensaio de imunoadsorção enzimática |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1016/j.jcvp.2022.100101 |
| URI: | http://hdl.handle.net/1843/81548 |
| Issue Date: | Aug-2022 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S2667038022000400?via%3Dihub |
| metadata.dc.relation.ispartof: | Journal of clinical virology plus |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19.pdf | 985.71 kB | Adobe PDF | View/Open |
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