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3. Artigo de Periódico

Type:	Artigo de Periódico
Title:	Allelic loss in amalgam-associated oral lichenoid lesions compared to oral lichen planus and mucosa
Authors:	Leonardo Nogueira Rodrigues Ricardo Santiago Gomez Vanessa de Fátima Bernardes Sílvia Ferreira de Sousa Raissa Cristina Costa Silva Mauro Henrique Guimarães Abreu Fábio Ramôa Pires Ricardo Alves de Mesquita Luciana Bastos Rodrigues Luiz de Marco Carolina Cavaliéri Gomes
Abstract:	Background: The amalgam-associated oral lichenoid lesion (AAOLL) shows clinical and histopathological features similar to oral lichen planus (OLP). Molecular researches to improve knowledge of pathogenesis and clinical behavior of AAOLL are still scarce. Objective: We investigated for the first time the use of loss of heterozygosity (LOH) as a molecular approach for genetic characterization of AAOLL in comparison with OLP and evaluated the cell proliferation index. Materials and methods: The sample comprised nine AAOLLs, 10 OLPs, and eight NOMs matched by patients' gender and age. LOH was assessed using polymorphic microsatellite markers at chromosomes 9p (D9S157, D9S162, D9S171), 11q (D11S1369), and 17p (TP53, AFM238WF2). Cell proliferation was assessed by immunohistochemical expression of Ki-67 (MIB-1). The association between LOH and Ki-67 was investigated. Results: Loss of heterozygosity occurred in 5/9 AAOLLs and in 2/10 OLPs in at least one marker each, while NOM showed no LOH. Cell proliferation index in AAOLL ranged from 2 to 23%. There was no association between cell proliferation and LOH, independent of the marker. Conclusion: Our study shows that the profile of molecular changes in AAOLL and OLP, evaluated by LOH and Ki-67 expression, is similar. Additional studies including larger samples should be performed to confirm or to refute our findings. Keywords: amalgam-associated oral lichenoid lesion; cell proliferation index; loss of heterozygosity; oral lichen planus.
Subject:	Loss of heterozygosity Lichen planus, oral Cell proliferation Ki-67 antigen Evaluation study
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ENF - DEPARTAMENTO DE NUTRIÇÃO FAO - DEPARTAMENTO DE CLÍNICA ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.1111/odi.12627
URI:	http://hdl.handle.net/1843/82724
Issue Date:	May-2017
metadata.dc.url.externa:	https://onlinelibrary.wiley.com/doi/10.1111/odi.12627
metadata.dc.relation.ispartof:	Oral Diseases
Appears in Collections:	Artigo de Periódico

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