Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/83011
Type: Artigo de Periódico
Title: Actinomyces israelii in radicular cysts: a molecular study
Authors: Nathália Rodrigues Gomes
Marina Gonçalves Diniz
Thais dos Santos Fontes Pereira
Carlos Estrela
Luiz de Macedo Farias
Bruno Augusto Benevenuto de Andrade
Carolina Cavaliéri Gomes
Ricardo Santiago Gomez
Abstract: Objective: To investigate whether the microscopic filamentous aggregates observed in radicular cysts are associated with the molecular identification of Actinomyces israelii. Moreover, to verify whether this bacterium can be detected in radicular cyst specimens not presenting aggregates. Study design: Microscopic colonies suggestive of Actinomyces were found in 8 out of 279 radicular cyst samples (case group). The case and control groups (n = 12; samples without filamentous colonies) were submitted to the semi-nested polymerase chain reaction to test the presence of A israelii. DNA sequencing was performed to validate polymerase chain reaction results. Results: Two and 3 samples in the case and control groups, respectively, did not present a functional genomic DNA template and were excluded from the study. A israelii was identified in all samples of the case group and in 3 out of 9 samples of the control group. Conclusions: Although A israelii is more commonly identified in radicular cysts presenting filamentous aggregates, it also appears to be detected in radicular cysts without this microscopic finding.
Subject: Radicular cyst
Bacteria
Association
language: eng
metadata.dc.publisher.country: Brasil
Publisher: Universidade Federal de Minas Gerais
Publisher Initials: UFMG
metadata.dc.publisher.department: FAO - DEPARTAMENTO DE CLÍNICA
ICB - DEPARTAMENTO DE MICROBIOLOGIA
ICB - DEPARTAMENTO DE MORFOLOGIA
ICB - DEPARTAMENTO DE PATOLOGIA
Rights: Acesso Restrito
metadata.dc.identifier.doi: https://doi.org/10.1016/j.oooo.2017.02.006
URI: http://hdl.handle.net/1843/83011
Issue Date: May-2017
metadata.dc.url.externa: https://www.sciencedirect.com/science/article/pii/S2212440317300652?via%3Dihub
metadata.dc.relation.ispartof: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Appears in Collections:Artigo de Periódico

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