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http://hdl.handle.net/1843/83317| Type: | Artigo de Periódico |
| Title: | DNA methylation patterns of genes related to immune response in the different clinical forms of oral lichen planus |
| Authors: | Aline Fernanda Cruz Renata Gonçalves de Resende Júlio César Tanos de Lacerda Nubia Braga Pereira Leonardo Augusto de Melo Marina Gonçalves Diniz Carolina Cavalieri Gomes Ricardo Santiago Gomez |
| Abstract: | Background: The oral lichen planus is a chronic inflammatory disease. Although its aetiology is not well understood, the role of T lymphocytes in its inflammatory events is recognised. Identifying the epigenetic mechanisms involved in the pathogenesis of this immune-mediated condition is fundamental for understanding the inflammatory reaction that occurs in the disease. The purpose of this work was to evaluate the methylation pattern of 21 immune response-related genes in the different clinical forms of oral lichen planus. Methods: A cross-sectional study was performed to analyse the DNA methylation patterns in three distinct groups of oral lichen planus: (i) reticular/plaque lesions; (ii) erosive lesions; (iii) normal oral mucosa (control group). After DNA extraction from biopsies, the samples were submitted to digestions by methylation-sensitive and methylation-dependent enzymes and double digestion. The relative percentage of methylated DNA for each gene was provided using real-time polymerase chain reaction arrays. Results: Hypermethylation of the STAT5A gene was observed only in the control group (59.0%). A higher hypermethylation of the ELANE gene was found in reticular/plaque lesions (72.1%) compared to the erosive lesions (50.0%). Conclusion: Our results show variations in the methylation profile of immune response-related genes, according to the clinical type of oral lichen planus after comparing with the normal oral mucosa. Further studies are necessary to validate these findings using gene expression analysis. |
| Subject: | Epigenomics Inflammation Lichen planus Methylation Lichen planus, oral Genes Dental plaque Tooth erosion Immunity |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE CLÍNICA ICB - DEPARTAMENTO DE MORFOLOGIA ICB - DEPARTAMENTO DE PATOLOGIA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1111/jop.12645 |
| URI: | http://hdl.handle.net/1843/83317 |
| Issue Date: | Jan-2018 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/10.1111/jop.12645 |
| metadata.dc.relation.ispartof: | Journal of Oral Pathology & Medicine |
| Appears in Collections: | Artigo de Periódico |
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