Analise das populacoes de celulas de langerhans e linfocitaria em lesoesescamosas intra-epiteliais cervicais e invasoras do colo uterino

Abstract

Squamous Intraepithelial Lesions (SILs) are pre-malignant cervical lesions, which can progress to cervical cancer (CC). Several studies demonstrated that high-risk (HR) Human Papillomaviruses (HPVs) are involved with the development of cervical premalignant/ malignant conditions, in addition to the abolishment of an effective Immuneresponse. Langerhans cells (LC) are considered the first and most important cells responsible for the recognition, processing and presenting-antigen in mucosa, including the cervical one. Whereas T cells require the transportation and processing of antigens,their functional dependence of CL is absolute requirement for them to initiate and perform their duties as effector cells. This study was focused on performing in histological sections of HPV-induced cervical SILs, the analysis of (i) HPV DNA presence and eight oncogenic types through Nested and Hemi-nested PCR technique (ii) the LC density bymeans of Indirect Immunofluorescence technique, and (iii) the CD4+, CD8+ and granzyme B+ subpopulations by Immunohistochemical staining. By analyzing the LC density ascertained by four distinct markers (S-100, Langerin, CD1a and CD83), a clear decrease of Langerin+ cells number in SIL lesions versus the normal epithelium was observed. Additionally, it was verified a significant increasing in the number of S100,CD1a, CD83 (LC), CD8, CD4 and granzyme B (immune-competent) positive cells along with the severity of cervical disease, when compared with the normal group. A higher quantity of CD1a+,CD83+ (LC) and CD8+ and granzyme B+-lymphocytes was observed of HPV+ and HPV16/18+ tissue/samples when compared to normal cervix. A severe decrease in LC and lymphocytes was observed in CINIII samples from those HPV+ and HPV16/18+ group. HPV 16 was the most prevalent in the samples studied, being present in 50% of CINIII samples and 60% of CC samples. 84% of CINIII samples had multiple infections. Thus, the increase of LC number through the distinct SIL degrees might beassociated with a possible increasing of LC migration to the site of cervical injury and recruitment of lymphocyte population in an attempt to control hrHPVs associated cervical lesion development.