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http://hdl.handle.net/1843/BUBD-AELNYK
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DC Field | Value | Language |
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dc.contributor.advisor1 | Walderez Ornelas Dutra | pt_BR |
dc.contributor.advisor-co1 | Annamaria Ravara Vago | pt_BR |
dc.contributor.referee1 | Renata Toscano Simões | pt_BR |
dc.contributor.referee2 | Juliana de Assis Silva Gomes Estanislau | pt_BR |
dc.contributor.referee3 | Claudia Rocha Carvalho | pt_BR |
dc.contributor.referee4 | Márcia Antoniarizi Michelin | pt_BR |
dc.creator | Daniele de Souza Camargos | pt_BR |
dc.date.accessioned | 2019-08-10T16:44:56Z | - |
dc.date.available | 2019-08-10T16:44:56Z | - |
dc.date.issued | 2015-09-29 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/1843/BUBD-AELNYK | - |
dc.description.abstract | Squamous Intraepithelial Lesions (SILs) are pre-malignant cervical lesions, which can progress to cervical cancer (CC). Several studies demonstrated that high-risk (HR) Human Papillomaviruses (HPVs) are involved with the development of cervical premalignant/ malignant conditions, in addition to the abolishment of an effective Immuneresponse. Langerhans cells (LC) are considered the first and most important cells responsible for the recognition, processing and presenting-antigen in mucosa, including the cervical one. Whereas T cells require the transportation and processing of antigens,their functional dependence of CL is absolute requirement for them to initiate and perform their duties as effector cells. This study was focused on performing in histological sections of HPV-induced cervical SILs, the analysis of (i) HPV DNA presence and eight oncogenic types through Nested and Hemi-nested PCR technique (ii) the LC density bymeans of Indirect Immunofluorescence technique, and (iii) the CD4+, CD8+ and granzyme B+ subpopulations by Immunohistochemical staining. By analyzing the LC density ascertained by four distinct markers (S-100, Langerin, CD1a and CD83), a clear decrease of Langerin+ cells number in SIL lesions versus the normal epithelium was observed. Additionally, it was verified a significant increasing in the number of S100,CD1a, CD83 (LC), CD8, CD4 and granzyme B (immune-competent) positive cells along with the severity of cervical disease, when compared with the normal group. A higher quantity of CD1a+,CD83+ (LC) and CD8+ and granzyme B+-lymphocytes was observed of HPV+ and HPV16/18+ tissue/samples when compared to normal cervix. A severe decrease in LC and lymphocytes was observed in CINIII samples from those HPV+ and HPV16/18+ group. HPV 16 was the most prevalent in the samples studied, being present in 50% of CINIII samples and 60% of CC samples. 84% of CINIII samples had multiple infections. Thus, the increase of LC number through the distinct SIL degrees might beassociated with a possible increasing of LC migration to the site of cervical injury and recruitment of lymphocyte population in an attempt to control hrHPVs associated cervical lesion development. | pt_BR |
dc.description.resumo | O carcinoma de celulas escamosas da cervice uterina e precedido por uma serie de modificacoes do epitelio original, que constituem as neoplasias intra - epiteliais cervicais (NIC), transformacoes progressivas que podem evoluir a lesoes invasoras. Estudos demonstram que os Papilomavirus Humano (HPVs) de alto risco estao intimamente associados ao desenvolvimento de processos celulares malignos e nasubversao da resposta imune na cervice uterina. As celulas de Langerhans (CL) tem-se mostrado as primeiras celulas responsaveis pelas funcoes de reconhecimento, processamento e apresentacao de antigeno em mucosas. Tais celulas representam absoluto requerimento para que Linfocitos T possam iniciar e exercer as suas funcoes como celulas efetoras. Dessa forma, este trabalho teve como objetivo efetuar a analise (i) da presenca do DNA do HPV e de oitos tipos oncogenicos por meio da tecnica de Nested e Hemi-nested PCR, (ii) da densidade de celulas de Langerhans no epitelio de amostras de Lesoes Escamosas Intra-epiteliais (LEIs) e invasoras do colo uterino induzidas pela presenca do HPV, por meio da tecnica de Imunofluorescencia indireta e(iii) das subpopulacoes de linfocitos T CD4+, CD8+ e de celulas granzima B+ no epitelio e no estroma, pela tecnica de Imunohistoquimica. A analise da densidade de LC determinada por quatro marcadores diferentes (S100, Langerina, CD1a e CD83) revelouqueda no numero de celulas Langerina+ em amostras com lesoes em relacao ao epitelio normal. No entanto, verificou-se um aumento significativo no numero de celulas de Langerhans S100, CD1a, CD83 positivas e de celulas imuno-competentes, CD8, CD4 e granzima B positivas, em LEIs e cancer, quando comparado com o grupo normal. Observou-se um aumento na densidade de CL (CD1a+, CD83+) e de celulas CD8+ e granzima B+ em amostras de NICII quando comparado as amostras controles, tanto no grupo de amostras HPV+ quanto nas amostras HPV16/18+. Porem, observou-se quedaexpressiva no numero de CL e linfocitos em lesoes de NICIII, com a maioria dos marcadores utilizados. O HPV16 foi o mais prevalente nas amostras estudadas, estando presente em 50% das amostras de NICIII e 60% das amostras de cancer. 84% das amostras de NICIII apresentaram multiplas infeccoes. Assim, o aumento do numero deCL atraves dos graus distintos de lesao pode estar associado com um possivel aumento da migracao de CL para o local da lesao cervical e recrutamento de populacoes de linfocitos, na tentativa de controlar LEIs associadas com HPVs de alto risco. | pt_BR |
dc.language | Português | pt_BR |
dc.publisher | Universidade Federal de Minas Gerais | pt_BR |
dc.publisher.initials | UFMG | pt_BR |
dc.rights | Acesso Aberto | pt_BR |
dc.subject | Biologia Celular | pt_BR |
dc.subject.other | Neoplasias do colo do útero | pt_BR |
dc.subject.other | Resposta imune | pt_BR |
dc.subject.other | Células de Langerhans | pt_BR |
dc.subject.other | Biologia celular | pt_BR |
dc.subject.other | Papillomaviridae | pt_BR |
dc.subject.other | Carcinoma de celulas escamosas | pt_BR |
dc.subject.other | Células dendríticas | pt_BR |
dc.title | Analise das populacoes de celulas de langerhans e linfocitaria em lesoesescamosas intra-epiteliais cervicais e invasoras do colo uterino | pt_BR |
dc.type | Tese de Doutorado | pt_BR |
Appears in Collections: | Teses de Doutorado |
Files in This Item:
File | Description | Size | Format | |
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tese_daniele_de_souza_camargos.pdf | 5.18 MB | Adobe PDF | View/Open |
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