Avaliação do Charm Cow Side II Test e Charm Blue Yellow II Test para a detecção de resíduos de antimicrobianos em leite

Abstract

The milk supply chain is an important stage in Brazilian agribusiness. Therefore, it is necessary to have strict quality control at all stages of production, including the use of antimicrobials in animals, which may generate residues in milk, causing damages to producer, industries andconsumer health. Two kits based on microbial inhibition (Charm® Cow Side II Test and Charm® Blue Yellow II Test) for detection of antimicrobial residues in milk in different concentrations were evaluated. The ability of both to detect the tetracycline group in a lower concentration of that considered by Brazilian law was also evaluated. Milk samples wereinoculated with standard solutions of 23 different antimicrobial agents and metabolites with a solution of one of antimicrobials (ceftiofur) in two different concentrations: the lower limit of detection stated by the manufacturer (Level 1) and the maximum residue limit (MRL) (Level 2)established by the legislation. Both kits were effective in detecting most of antimicrobials tested in two concentrations, including metabolites of ceftiofur. It is necessary to review information from the manufacturer of the kit Charm® Cow Side II Test for the detection of residues of oxacillin, penicillin G, spiramycin, and sulfonamides and Charm® Blue Yellow II Test on the detection of erythromycin, cloxacillin, sulfadiazine, tylosin, and penicillin G in milk. Both kits detected residues of tetracyclines given the MRLs required by the Brazilian law and can be safely used for monitoring these drugs in milk. Keywords: milk, antimicrobials, residues, microbial inhibition