Avaliação da presença de Leishmania sp. em ossos autoclavados ou armazenados em glicerol para enxertia cortical

Abstract

The aim of this study was to evaluate the effect of autoclaving and storage in 85% glycerol in cortical bone of dogs infected by Leishmania sp., and evaluate their morphological and mechanical characteristics. We used 43 cadavers of dogs with leishmaniasis and 11 cadavers of dogs free from the disease. From the infected animals were harvested 43 diaphysis of the right femur and 43 corresponding bone marrow samples. The shaft was divided in two, one part fragmented into small particles and the other split into two fragments of about 1cm in length for mechanical testing and morphometric study. The fragmentary was subjected to culture the parasite before and after autoclaving and storage in glycerol. Samples of the diaphysis of negative dogs were also subjected to morphometric and mechanical ratings. Both treatments were effective in preventing the growth of the parasite in vitro. The mechanical tests showed no statistical difference in deformation tension or the elastic modulus by mechanical compression between the infected and uninfected groups. There was a significant increase in the morphometric analysis of the percentage of cortical osteocytes in infected animals. It was concluded that the treatments are effective in eliminating the parasite and there is no change in strength of the bone due to infection, therefore beeing viable their use in grafting. More studies are needed on the mechanisms involved in the increase of osteocytes in cortical tissue