Splenic implant preservation after conservation in lactated ringer solution
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Universidade Federal de Minas Gerais
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Artigo de periódico
Título alternativo
Preservação de implante esplênico autógeno após conservação em solução de Ringer-lactato
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Membros da banca
Resumo
Objective: to evaluate the morphology and function of autogenous splenic tissue implanted in the greater omentum, 24 hours after
storage in Ringer-lactate solution. Methods: we divided 35 male rats into seven groups (n=5): Group 1: no splenectomy; Group 2: total
splenectomy without implant; Group 3: total splenectomy and immediate autogenous implant; Group 4: total splenectomy, preservation
of the spleen in Ringer-lactate at room temperature, then sliced and implanted; Group 5: total splenectomy, spleen sliced and preserved
in Ringer-lactate at room temperature before implantation; Group 6: total splenectomy with preservation of the spleen in Ringer lactate at 4°C and then sliced and implanted; Group 7: total splenectomy and the spleen sliced for preservation in Ringer-lactate at
4°C before implantation. After 90 days, we performed scintigraphic studies with Tc99m-colloidal tin (liver, lung, spleen or implant and
clot), haematological exams (erythrogram, leucometry, platelets), biochemical dosages (protein electrophoresis) and anatomopathological studies. Results: regeneration of autogenous splenic implants occurred in the animals of the groups with preservation of the spleen at 4ºC. The uptake of colloidal tin was higher in groups 1, 3, 6 and 7 compared with the others. There was no difference in hematimetric values in the seven groups. Protein electrophoresis showed a decrease in the gamma fraction in the group of splenectomized animals in relation to the operated groups. Conclusion: the splenic tissue preserved in Ringer-lactate solution at 4ºC maintains its morphological structureand allows functional recovery after being implanted on the greater omentum
Abstract
Assunto
Spleen, Implants, Experimental, Trauma and Stressor Related Disorders, Organ Preservation
Palavras-chave
Spleen, Implants, Experimental, Trauma and Stressor Related Disorders, Organ Preservation
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Endereço externo
https://doi.org/10.1590/0100-6991e-20181346