Splenic implant preservation after conservation in lactated ringer solution

dc.creatorArgos Soares Matos Filho
dc.creatorAndy Petroianu
dc.creatorValbert Nascimento Cardoso
dc.creatorPaula Vieira Teixeira Vidigal
dc.date.accessioned2024-01-22T19:25:18Z
dc.date.accessioned2025-09-09T01:33:36Z
dc.date.available2024-01-22T19:25:18Z
dc.date.issued2018
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1590/0100-6991e-20181346
dc.identifier.issn01006991
dc.identifier.urihttps://hdl.handle.net/1843/63211
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofRevista do Colégio Brasileiro de Cirurgiões
dc.rightsAcesso Aberto
dc.subjectSpleen
dc.subjectImplants, Experimental
dc.subjectTrauma and Stressor Related Disorders
dc.subjectOrgan Preservation
dc.subject.otherSpleen
dc.subject.otherImplants, Experimental
dc.subject.otherTrauma and Stressor Related Disorders
dc.subject.otherOrgan Preservation
dc.titleSplenic implant preservation after conservation in lactated ringer solution
dc.title.alternativePreservação de implante esplênico autógeno após conservação em solução de Ringer-lactato
dc.typeArtigo de periódico
local.citation.epagee1354
local.citation.issue1
local.citation.spagee1346
local.citation.volume45
local.description.resumoObjective: to evaluate the morphology and function of autogenous splenic tissue implanted in the greater omentum, 24 hours after storage in Ringer-lactate solution. Methods: we divided 35 male rats into seven groups (n=5): Group 1: no splenectomy; Group 2: total splenectomy without implant; Group 3: total splenectomy and immediate autogenous implant; Group 4: total splenectomy, preservation of the spleen in Ringer-lactate at room temperature, then sliced and implanted; Group 5: total splenectomy, spleen sliced and preserved in Ringer-lactate at room temperature before implantation; Group 6: total splenectomy with preservation of the spleen in Ringer lactate at 4°C and then sliced and implanted; Group 7: total splenectomy and the spleen sliced for preservation in Ringer-lactate at 4°C before implantation. After 90 days, we performed scintigraphic studies with Tc99m-colloidal tin (liver, lung, spleen or implant and clot), haematological exams (erythrogram, leucometry, platelets), biochemical dosages (protein electrophoresis) and anatomopathological studies. Results: regeneration of autogenous splenic implants occurred in the animals of the groups with preservation of the spleen at 4ºC. The uptake of colloidal tin was higher in groups 1, 3, 6 and 7 compared with the others. There was no difference in hematimetric values in the seven groups. Protein electrophoresis showed a decrease in the gamma fraction in the group of splenectomized animals in relation to the operated groups. Conclusion: the splenic tissue preserved in Ringer-lactate solution at 4ºC maintains its morphological structureand allows functional recovery after being implanted on the greater omentum
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGAL
local.publisher.departmentMED - DEPARTAMENTO DE CIRURGIA
local.publisher.initialsUFMG
local.url.externahttps://doi.org/10.1590/0100-6991e-20181346

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