Seleção e caracterização de peptídeos miméticos e anticorpos monoclonais (scFv) para Campylobacter fetus subsp. venerealis por Phage display

Abstract

Bovine genital Campylobacteriosis is a venereal disease that causes reproductive problems in herds, with great importance in countries where there is widespread use of natural service. Phage display is a technique that applies bacteriophage libraries that displays peptides fused to the viral capsid proteins in selections against biological target. The aim of the present study was to select mimetic peptides to Campylobacter fetus subsp. venerealis and recombinant monoclonal antibody fragments (scFv). Library of conformational seven aminoacids peptides and scFv library constructed from tumor cells were uses. Five peptides was considered mimetics to C. fetus subsp. venerealis NCTC 10354 - P3, and hyperimmune sera were produced in mice and performed immunoassays with bacterial antigens and samples of cervical-vaginal mucus of heifers. Eight clones expressing scFv antibodies reactive against C. fetus subsp. venerealis NCTC 10354 - P3 were selected and screened by ELISA. Among the mimetic peptides, two were considered promising (ALASLPL and LSYLFPP) due to the reaction of hyperimmune serum against bacteria and synthetic peptides with cervical-vaginal mucus. ScFv antibodies showed cross-reactivity between bacteria of the genus Campylobacter and two clones (3H6 and 4G7) showed low reactivity against Escherichia coli and Staphylococcus aureus.