Biocompatibility and immunohistochemical evaluation of a new calcium silicate-based cement, bio-c pulpo
Carregando...
Data
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Minas Gerais
Descrição
Tipo
Artigo de periódico
Título alternativo
Primeiro orientador
Membros da banca
Resumo
AimTo evaluate the inflammatory response andability to induce mineral deposition through histologi-cal and immunohistochemical analysis for osteocalcin(OCN), osteopontin (OPN) and bone sialoprotein (BSP)of a new calcium silicate-based cement, Bio-C Pulpo(Angelus), compared to white mineral trioxide aggre-gate (White MTA-Ang) (Angelus).MethodologyPolyethylene tubes containing Bio-CPulpo and White MTA-Ang as well as empty tubeswere implanted into the dorsal connective tissue of30 Wistar rats, which were arranged in five groupsaccording to the period of analysis: 7, 15, 30, 60and 90 days. After each experimental period, thetubes with surrounding tissue were removed and his-tologically processed to be analysed usinghaematoxylin–eosin and immunohistochemistry forthe detection of OCN, OPN and BSP. The data werestatistically analysed (Friedman’s test) at a 5% signifi-cance level.ResultsThe inflammatory response observed withBio-C Pulpo and White MTA-Ang was greater after 7and 15 days and decreased from 30 days onwards.No significant difference was found between the con-trol, Bio-C Pulpo and White MTA-Ang at the differentperiods of analysis (P>0.05). The immunolabellingfor OCN, OPN and BSP was more intense for Bio-CPulpo and White MTA-Ang after 60 and 90 days,but there was no difference between Bio-C Pulpo andWhite MTA-Ang at the different periods of analysis(P>0.05).ConclusionBio-C Pulpo is biocompatible andinduces immunolabelling of osteogenic markers suchas OCN, OPN and BSP similar to White MTA-Ang.
Abstract
Assunto
Osteocalcin, Osteopontin, Materials testing, Pulpotomy
Palavras-chave
Biocompatibility, Bone sialoprotein, Osteocalcin, Osteopontin, pulpotomy
Citação
Departamento
Curso
Endereço externo
https://onlinelibrary.wiley.com/doi/10.1111/iej.13052