Evaluation of chemokines and receptors in gnotobiotic root canal infection by f. nucleatum and e. faecalis
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Resumo
The present study aims to evaluate the longitudinal effects of
induced experimental infections in gnotoxenic animals on the expression
of inflammatory chemokines and their receptors in periradicular tissues.
The null hypothesis tested was that Enterococcus faecalis and Fusobacterium
nucleatum had no effect on CCR5, CCL5, CXCL10, CCL2/MCP-1, CXCR2
and CCR1 expression. Two groups of five animals (n = 5) aged between
8 and 12 weeks were used in this study. The animals were anaesthetized,
and coronary access was performed in the first molar on the right and left
sides. Microorganisms were inoculated into the left molar, and the right
molar was sealed without contamination to function as a control. Animals
were sacrificed 7 and 14 days after infection, and periapical tissues were
collected. The cytokine mRNA expression levels were assessed using realtime PCR. The chemokine mRNA expression levels demonstrated that
the experimental infection was capable of inducing increased chemokine
expression on day 7 compared to that on day 14, except for CCR5 and
CCL5, which showed no changes. The gnotoxenic animal model proved
to be effective and allowed evaluation of the immune response against
a known infection. Additionally, this study demonstrates that gene
expression of chemokines and their receptors against the experimental
infection preferentially prevailed during the initial phase of induction of
the periradicular alteration (i.e., on day 7 post-infection)
Abstract
Assunto
Germ-free life, Periapical Diseases, Chemokines, Infections
Palavras-chave
Germ-free life, Chemonikes, Periapical Diseases, Microbiology
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https://www.scielo.br/j/bor/a/63hGYMdnhYmRmSbfyv4ZswB/?lang=en