Evaluation of chemokines and receptors in gnotobiotic root canal infection by f. nucleatum and e. faecalis

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dc.creatorCaroline Christine Santa Rosa
dc.creatorMarcela Marçal Thebit
dc.creatorKamilla Faria Maciel
dc.creatorLuciana Carla Neves Brito
dc.creatorLeda Quércia Vieira
dc.creatorAntônio Paulino Ribeiro Sobrinho
dc.date.accessioned2022-09-24T16:55:30Z
dc.date.accessioned2025-09-08T23:58:19Z
dc.date.available2022-09-24T16:55:30Z
dc.date.issued2018-11-29
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doi10.1590/1807-3107bor-2018.vol32.0120
dc.identifier.issn18073107
dc.identifier.urihttps://hdl.handle.net/1843/45442
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBrazilian Oral Research
dc.rightsAcesso Aberto
dc.subjectGerm-free life
dc.subjectPeriapical Diseases
dc.subjectChemokines
dc.subjectInfections
dc.subject.otherGerm-free life
dc.subject.otherChemonikes
dc.subject.otherPeriapical Diseases
dc.subject.otherMicrobiology
dc.titleEvaluation of chemokines and receptors in gnotobiotic root canal infection by f. nucleatum and e. faecalis
dc.typeArtigo de periódico
local.citation.epage7
local.citation.spage1
local.citation.volume32
local.description.resumoThe present study aims to evaluate the longitudinal effects of induced experimental infections in gnotoxenic animals on the expression of inflammatory chemokines and their receptors in periradicular tissues. The null hypothesis tested was that Enterococcus faecalis and Fusobacterium nucleatum had no effect on CCR5, CCL5, CXCL10, CCL2/MCP-1, CXCR2 and CCR1 expression. Two groups of five animals (n = 5) aged between 8 and 12 weeks were used in this study. The animals were anaesthetized, and coronary access was performed in the first molar on the right and left sides. Microorganisms were inoculated into the left molar, and the right molar was sealed without contamination to function as a control. Animals were sacrificed 7 and 14 days after infection, and periapical tissues were collected. The cytokine mRNA expression levels were assessed using realtime PCR. The chemokine mRNA expression levels demonstrated that the experimental infection was capable of inducing increased chemokine expression on day 7 compared to that on day 14, except for CCR5 and CCL5, which showed no changes. The gnotoxenic animal model proved to be effective and allowed evaluation of the immune response against a known infection. Additionally, this study demonstrates that gene expression of chemokines and their receptors against the experimental infection preferentially prevailed during the initial phase of induction of the periradicular alteration (i.e., on day 7 post-infection)
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/bor/a/63hGYMdnhYmRmSbfyv4ZswB/?lang=en

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