KRAS mutations drive adenomatoid odontogenic tumor
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Membros da banca
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Objective:
KRAS is the most frequently mutated onco
gene in human neoplasms and we have previously reported
KRAS p.G12V mutations in adenomatoid odontogenic tumors
(AOT). We aimed to expand this cohort of samples and to test
the association of KRAS mutations with clinical and histopatho
logical parameters. A convenience sample of 30 AOT cases was
included in the study. The hotpot KRAS p.G12V mutation was
assessed by TaqMan allele-specific qPCR and codon 12 was
direct sequenced. Clinical information obtained included patients
age, tumor site, association of the lesion with impacted teeth and
clinical tumor size. In addition, tumor capsule thickness was
evaluated by morphometric analysis. Statistical analysis was car
ried out to test the association of KRAS codon 12 mutations with
clinico-pathological parameters.
Findings:
Molecular results confirmed KRAS p.G12V
mutation in 14/23 cases, and p.G12R in 1/23. Eight cases were
wild-type and samples from 7 cases failed amplification. Codon
12 mutations were not associated with any of the clinicopatho
logical parameters tested (p>0.05).
Conclusion:
AOTshow high frequency of KRAS codon
12 mutations (15/23, 65%), which occur irrespectively of
patients’ age, tumor location, association with impacted teeth,
tumor clinical size or histopathological capsule thickness.
Abstract
Assunto
Association, Mutation, Codon, Oncogenes, Evaluation study
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Endereço externo
https://www.oooojournal.net/article/S2212-4403(19)30327-X/fulltext