KRAS mutations drive adenomatoid odontogenic tumor

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dc.creatorBruna Pizziolocoura
dc.creatorAline Carvalho Batista
dc.creatorMarina Gonçalves Diniz
dc.creatorRicardo Santiago Gomez
dc.creatorSílvia Ferreira de Sousa
dc.creatorVanessa de Fátima Bernardes
dc.creatorJosiane Alves França
dc.creatorHelder Antonio Rebelo Pontes
dc.creatorDanyel Elias da Cruz Perez
dc.creatorCarolina Cavaliéri Gomes
dc.creatorRicardo Luiz Cavalcanti de Albuquerque Junior
dc.creatorManoela Domingues Martins
dc.date.accessioned2025-07-29T18:19:31Z
dc.date.accessioned2025-09-08T23:14:50Z
dc.date.available2025-07-29T18:19:31Z
dc.date.issued2019-07
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.issn2212-4411
dc.identifier.urihttps://hdl.handle.net/1843/83923
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofOral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
dc.rightsAcesso Restrito
dc.subjectAssociation
dc.subjectMutation
dc.subjectCodon
dc.subjectOncogenes
dc.subjectEvaluation study
dc.titleKRAS mutations drive adenomatoid odontogenic tumor
dc.typeArtigo de periódico
local.citation.epagee77
local.citation.issue1
local.citation.spagee77
local.citation.volume128
local.description.resumoObjective: KRAS is the most frequently mutated onco gene in human neoplasms and we have previously reported KRAS p.G12V mutations in adenomatoid odontogenic tumors (AOT). We aimed to expand this cohort of samples and to test the association of KRAS mutations with clinical and histopatho logical parameters. A convenience sample of 30 AOT cases was included in the study. The hotpot KRAS p.G12V mutation was assessed by TaqMan allele-specific qPCR and codon 12 was direct sequenced. Clinical information obtained included patients age, tumor site, association of the lesion with impacted teeth and clinical tumor size. In addition, tumor capsule thickness was evaluated by morphometric analysis. Statistical analysis was car ried out to test the association of KRAS codon 12 mutations with clinico-pathological parameters. Findings: Molecular results confirmed KRAS p.G12V mutation in 14/23 cases, and p.G12R in 1/23. Eight cases were wild-type and samples from 7 cases failed amplification. Codon 12 mutations were not associated with any of the clinicopatho logical parameters tested (p>0.05). Conclusion: AOTshow high frequency of KRAS codon 12 mutations (15/23, 65%), which occur irrespectively of patients’ age, tumor location, association with impacted teeth, tumor clinical size or histopathological capsule thickness.
local.identifier.orcidhttps://orcid.org/0000-0002-9987-5311
local.identifier.orcidhttps://orcid.org/0000-0002-2117-5593
local.identifier.orcidhttps://orcid.org/0000-0002-4212-1172
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0001-7820-4749
local.identifier.orcidhttps://orcid.org/0000-0003-0194-7434
local.identifier.orcidhttps://orcid.org/0000-0002-6005-783X
local.identifier.orcidhttps://orcid.org/0000-0002-7609-8804
local.identifier.orcidhttps://orcid.org/0000-0002-4591-4645
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.oooojournal.net/article/S2212-4403(19)30327-X/fulltext

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