Oleate hydratase in lactobacillus delbrueckii subsp. bulgaricus LBP UFSC 2230 catalyzes the reversible conversion between linoleic acid and ricinoleic acid

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dc.creatorGabriela Christina Kuhl
dc.creatorRicardo Ruiz Mazzon
dc.creatorBrenda Lee Simas Porto
dc.creatorTâmela Zamboni Madaloz
dc.creatorGuilherme Razzera Maciel
dc.creatorDaniel de Oliveira Patricio
dc.creatorKevin Linehan
dc.creatorGrace Ahern
dc.creatorHarsh Mathur
dc.creatorReynolds Paul Ross
dc.creatorCatherine Stanton
dc.creatorJuliano de Dea Lindner
dc.date.accessioned2024-12-06T18:54:53Z
dc.date.accessioned2025-09-09T00:26:27Z
dc.date.available2024-12-06T18:54:53Z
dc.date.issued2021-10-31
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1128/Spectrum.01179-21
dc.identifier.issn2165-0497
dc.identifier.urihttps://hdl.handle.net/1843/78511
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMicrobiology Spectrum
dc.rightsAcesso Aberto
dc.subjectBacterias produtoras de acido lactico
dc.subjectHidrogenação
dc.subjectLactobacilo
dc.subject.otherBiohydrogenation
dc.subject.otherHeterologous expression
dc.subject.otherHomology modeling
dc.subject.otherCis-9
dc.subject.otherTrans-11 CLA
dc.titleOleate hydratase in lactobacillus delbrueckii subsp. bulgaricus LBP UFSC 2230 catalyzes the reversible conversion between linoleic acid and ricinoleic acid
dc.typeArtigo de periódico
local.citation.issue2
local.citation.spagee0117921
local.citation.volume9
local.description.resumoConjugated linoleic acid (CLA) has been the subject of numerous studies in recent decades because of its associated health benefits. CLA is an intermediate product of the biohydrogenation pathway of linoleic acid (LA) in bacteria. Several bacterial species capable of efficiently converting LA into CLA have been widely reported in the literature, among them Lactobacillus delbrueckii subsp. bulgaricus LBP UFSC 2230. Over the last few years, a multicomponent enzymatic system consisting of three enzymes involved in the biohydrogenation process of LA has been proposed. Sequencing the genome of L. delbrueckii subsp. bulgaricus LBP UFSC 2230 revealed only one gene capable of encoding an oleate hydratase (OleH), unlike the presence of multiple genes typically found in similar strains. This study investigated the biological effect of the OleH enzyme of L. delbrueckii subsp. bulgaricus LBP UFSC 2230 on the hydration of LA and dehydration of ricinoleic acid (RA) and its possible role in the production of CLA. The OleH was cloned, expressed, purified, and characterized. Fatty acid measurements were made by an internal standard method using a gas chromatography coupled flame ionization detector (GC-FID) system. It was found that the enzyme is a hydratase/dehydratase, leading to a reversible transformation between LA and RA. In addition, the results showed that L. delbrueckii subsp. bulgaricus LBP UFSC 2230 OleH protein plays a role in stress tolerance in Escherichia coli. In conclusion, the OleH of L. delbrueckii subsp. bulgaricus LBP UFSC 2230 catalyzes the initial stage of saturation metabolism of LA, although it has not converted the substrates directly into CLA.
local.identifier.orcidhttps://orcid.org/0000-0002-8913-9177
local.identifier.orcidhttps://orcid.org/0000-0002-8034-6284
local.identifier.orcidhttps://orcid.org/0000-0003-3469-0284
local.identifier.orcidhttps://orcid.org/0000-0001-8087-6309
local.identifier.orcidhttps://orcid.org/0000-0002-1448-3037
local.identifier.orcidhttps://orcid.org/0000-0001-8318-3778
local.identifier.orcidhttps://orcid.org/0000-0003-1889-5984
local.identifier.orcidhttps://orcid.org/0000-0003-4876-8839
local.identifier.orcidhttps://orcid.org/0000-0002-6724-7011
local.identifier.orcidhttps://orcid.org/0000-0002-6191-8910
local.publisher.countryBrasil
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://journals.asm.org/doi/10.1128/spectrum.01179-21

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