Caga-positive helicobacter pylori strain containing three epiya c phosphorylation sites produces increase of g cell and decrease of d cell in experimentally infected gerbils (meriones unguiculatus)
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Universidade Federal de Minas Gerais
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Purpose: Human infection by Helicobacter pylori is associated with an increase in the number of gastrin producing G cells and a concomitant decrease of somatostatin-producing D cells. However, to our knowledge, changes in G and D cell numbers in response to infection with H. pylori CagA-positive strains containing different number of EPIYA-C phosphorylation sites have not been analyzed to date. Therefore,the aim of this study was to perform a quantitative analysis of the number of G and D cells in Mongolian gerbils challenged with H. pylori strains with different numbers of EPIYA-C motifs.Materials and methods: Mongolian gerbils were inoculated with isogenic H. pylori strains containing one to three phosphorylation sites. Mucosal fragments were evaluated by morphometry and immunohistochemistry using primary polyclonal rabbit anti-gastrin and anti-somatostatin antibodies. Positive cells
were counted using an image analyzer.Results: Forty-five days after infection, there was a decrease in the number of D cells and an increase inthe G/D cell ratio in the group with three EPIYA-C. Six months after infection, there was a progressive and
significant increase in the number of G cells and in the G/D cell ratio, with a concomitant decrease in thenumber of D cells, especially in the three EPIYA-C group.Conclusions: CagA-positive H. pylori strains containing a large number of EPIYA-C phosphorylation sites
induce a decrease in D cell number and an increase in G cell number and G/D ratio, which were correlated with the number of inflammatory cells of the lamina propria.
Abstract
Assunto
Helicobacter pylori, Células Secretoras de Gastrina, Células Secretoras de Somatostatina
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Helicobacter pylori, G cells, Células Secretoras de Somatostatina, Cag A
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https://www.sciencedirect.com/science/article/pii/S1896112616000158?via%3Dihub