Efeito do bloqueio do receptor mas em camundongos com inflamação pulmonar alérgica crônica, submetidos a treinamento aeróbio de moderada intensidade

Abstract

Asthma is a chronic inflammatory airway disease that involves multiple cells in particular T-lymphocytes, mast cells, eosinophils, neutrophils and epithelial cells. In the present study, we evaluated the participation of the angiotensin-(1-7) [Ang-(1-7)] receptor Mas, in the beneficial effects of aerobic training in an experimental model of asthma. Balb/ c mice were subjected to a protocol chronic asthma (OVA sensitization), infused with the Mas receptor antagonist, A779 (1µg/h), and trained 5x/week (moderate/ treadmill). The serum IgE levels were determined by ELISA, the airway remodelling by histology, infiltration of inflammatory cells by enzyme activity assay. Furthermore, the physical performance was determined by maximum effort test. The gene expression of PGC-1 α was determined by qRT-PCR, protein expression of the Mas receptor and fibronectin by Western blotting. The main results were, treatment of asthmatic and trained animals with A779 prevented the reduction in serum IgE levels, reduction in extracellular matrix deposition in the airways, reduction in the alveolar wall thickening, reduction in MPO enzyme activity. Protein expression of the Mas receptor in the lung was not altered. Physical training was confirmed by the increase in PGC1-α in control or asthmatic animals. These data suggest that Mas receptor activation is involved, at least in part, in the anti-inflammatory and anti-fibrotic effect of aerobic training animals subjected to an experimental model of chronic pulmonary lung inflammation.