Desenvolvimento de métodos baseados em cromatografia líquida acoplada à espectrometria de massas para determinação de micotoxinas e agrotóxicos em alimentos

Abstract

Analytical methods were developed for the determination of mycotoxins in vegetable milks, baby food and roasted coffee, by applying a procedure based on the QuEChERS extraction technique and the development of dSPE techniques with the use of multi-walled carbon nanotubes and graphene nanoplatelets. Quantification was performed using the ultra-efficient liquid chromatography technique coupled with sequential mass spectrometry and using an ESI ionization source. A metrological study was carried out for comparative evaluation between the main procedures for estimating uncertainty of analytical measurement, Bottom-up method and Top-down method, in an analytical method developed to control residues and contaminants in food using dilute and shoot extraction and analysis by liquid chromatography and sequential mass spectrometry. The developed methods were validated according to the guidelines pointed out by the EURACHEM guides, the Analytical Quality Assurance Manual, the Ministry of Agriculture, Livestock and Supply, and the European Union regulations 2002/657/EC, showing performance compatible with those demanded for control of residues and contaminants in food. With this work, it is understood that the development of analytical methodologies based on more dynamic and modern practices, associated with a deeper understanding of the metrological nature of the tests, can confer operational advantages of great value to the test laboratories, which, in the environment of food quality control samples, reflects a higher quality of products offered to consumers and competitiveness to national food producers, resulting in the strengthening of food security.