Human periodontal ligament- and gingiva-derived mesenchymal stem cells promote nerve regeneration when encapsulated in alginate/hyaluronic acid 3d scaffold
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Repair or regeneration of damaged nerves is still a challenging clinical task in reconstructive
surgeries and regenerative medicine. Here, we demonstrate that periodontal ligament stem cells
(PDLSCs) and gingival mesenchymal stem cells (GMSCs) isolated from adult human periodontal
and gingival tissues assume neuronal phenotype in vitro and in vivo via a subcutaneous
transplantation model in nude mice. PDLSCs and GMSCs were encapsulated in a three dimensional scaffold based on alginate and hyaluronic acid hydrogels capable of sustained release
of human nerve growth factor (NGF). We demonstrate that, the elasticity of the hydrogels affected the proliferation and differentiation of encapsulated MSCs within scaffolds. Moreover, we
observed that PDLSCs and GMSCs were stained positive for βIII-tubulin, while exhibiting high
levels of gene expression related to neurogenic differentiation (βIII-tubulin and GFAP) via qPCR.
Western blot analysis showed the importance of the elasticity of the matrix and the presence of
NGF in the neurogenic differentiation of encapsulated MSCs. In vivo, immunofluorescence
staining for neurogenic specific protein markers confirmed islands of dense positively stained
structures inside transplanted hydrogels. To our knowledge, this study is the first demonstration of
the application of PDLSCs and GMSCs as promising cell therapy candidates for nerve
regeneration
Abstract
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Hydrogels, Hyaluronic acid, Gingiva, Periodontics, Mesenchymal stem cells
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813692/