Human periodontal ligament- and gingiva-derived mesenchymal stem cells promote nerve regeneration when encapsulated in alginate/hyaluronic acid 3d scaffold

Página do item simplificado
dc.creatorSahar Ansari
dc.creatorIvana Márcia Alves Diniz
dc.creatorPatrícia Sarrion
dc.creatorChider Chen
dc.creatorAli Tamayol
dc.creatorWu
dc.creatorAlireza Moshaverinia
dc.date.accessioned2022-12-16T14:57:03Z
dc.date.accessioned2025-09-08T23:28:16Z
dc.date.available2022-12-16T14:57:03Z
dc.date.issued2017
dc.identifier.doi10.1002/adhm.201700670
dc.identifier.issn21922640
dc.identifier.urihttps://hdl.handle.net/1843/48139
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofAdvanced Healthcare Materials
dc.rightsAcesso Aberto
dc.subjectHydrogels
dc.subjectHyaluronic acid
dc.subjectGingiva
dc.subjectPeriodontics
dc.subjectMesenchymal stem cells
dc.titleHuman periodontal ligament- and gingiva-derived mesenchymal stem cells promote nerve regeneration when encapsulated in alginate/hyaluronic acid 3d scaffold
dc.typeArtigo de periódico
local.citation.spage2017
local.citation.volume2017
local.description.resumoRepair or regeneration of damaged nerves is still a challenging clinical task in reconstructive surgeries and regenerative medicine. Here, we demonstrate that periodontal ligament stem cells (PDLSCs) and gingival mesenchymal stem cells (GMSCs) isolated from adult human periodontal and gingival tissues assume neuronal phenotype in vitro and in vivo via a subcutaneous transplantation model in nude mice. PDLSCs and GMSCs were encapsulated in a three dimensional scaffold based on alginate and hyaluronic acid hydrogels capable of sustained release of human nerve growth factor (NGF). We demonstrate that, the elasticity of the hydrogels affected the proliferation and differentiation of encapsulated MSCs within scaffolds. Moreover, we observed that PDLSCs and GMSCs were stained positive for βIII-tubulin, while exhibiting high levels of gene expression related to neurogenic differentiation (βIII-tubulin and GFAP) via qPCR. Western blot analysis showed the importance of the elasticity of the matrix and the presence of NGF in the neurogenic differentiation of encapsulated MSCs. In vivo, immunofluorescence staining for neurogenic specific protein markers confirmed islands of dense positively stained structures inside transplanted hydrogels. To our knowledge, this study is the first demonstration of the application of PDLSCs and GMSCs as promising cell therapy candidates for nerve regeneration
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.initialsUFMG
local.url.externahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813692/

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Human periodontal ligament and gingiva derived mesenchymal stem cells promote nerve regeneration when encapsulated in alginate hyaluronic acid 3d scaffold.pdf
Tamanho:
1.89 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG